Human IL-6 ELISA Kit SHENTEK®

Description

SHENTEK® Human IL-6 ELISA Kit is intended for the determination of natural or recombinant human interleukin-6 (hIL-6) in biological samples, such as serum or cell culture supernatants.

Overview

This kit is based on a solid-phase enzyme-linked immunosorbent assay (ELISA) using a double-antibody sandwich technique to determine the amount of hIL-6 in biological samples. An hIL-6–specific capture antibody is pre-coated onto each well of the microtiter strips. Both calibration standards and test samples are added simultaneously to the wells, followed by incubation and washing. A biotinylated detection antibody is then introduced to bind the epitope of hIL-6, forming a sandwich complex that subsequently reacts with streptavidin-labeled HRP (SA-HRP). TMB substrate is added to the reaction, and HRP catalyzes its oxidation to produce a blue-colored product (maximum absorption at 655 nm). The enzymatic reaction is terminated with the stop solution, yielding a yellow-colored product (maximum absorption at 450 nm). The absorbance at 450 nm is positively correlated with the hIL-6 concentration in both the calibration standards and the test samples. The concentration of hIL-6 in the samples can be calculated using a dose–response curve.

Introduction

Specification