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Human IFG Protein, Human IFI Protein, Human IFN gamma Protein, Human Interferon Gamma Protein
$82.00
Sku: RP450-10UG
Bcakground
Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20–25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%–64% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37%–43% with cotton rat, mouse, and rat IFN-gamma.
IFN-gamma dimers bind to IFN-gamma RI (alpha subunits), which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for the ligand and the efficiency of signal transduction (4, 5).
IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up-regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7).
In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
Reference
1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Specifications
| Synonyms | Human IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma |
| Accession # | CAA31639 |
| Source | Human embryonic kidney cell, HEK293-derived human IFN-gamma protein |
| Gln24-Gln166 | |
| Predicted Moleucular weight | 16.8 kDa |
Components and Storage
| Formulation | Solution protein |
| Dissolved in sterile PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening. | |
| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice |
Quality
| Purity | > 95%, determined by SDS-PAGE |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method |
| Activity | Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis virus. |
| The EC50 for this effect is 0.10-0.70 ng/mL. |
SDS-PAGE
Gel filtration
Bioactivity
Documents
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