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Bcakground
Int erf ero n bet a (I F N - bet a ) , also known as fibroblast IF N, is a se creted, approximately 22 kDa member of the type I interferon family of molecules (1 ). Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IF N-be ta, but it can als o be produced by dendritic cells, macrophages, and endothelial cells in res pons e to pathogen exposur e (2). It is trans -criptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3). Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway (4-7). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis ( E AE ), a disease model of human multiple s clerosis (M S ) (8). Furth ermore, IF N-be ta has been shown to s uppress the Th 17 cell respons e in both MS and EAE and has commonly been used as a treatment for MS (9-13).
Reference
1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
4. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
5. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
6. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
7. Matikainen, S. et al. (1999) Blood 93:1980.
8. Teige, I. et al. (2003) J. Immunol. 170:4776.
9. Shinohara, M.L. et al. (2008) Immunity 29:68.
10.Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
11.Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
12. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
13. Inoue M. and M.L. Shinohara (2013) Immunology 139:11.
Specifications
| Synonyms | Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta |
| Accession # | P01574 |
| Source | Human embryonic kidney cell, HEK293-derived human IFN-beta protein |
| Predicted Moleucular weight | Met22-Asn187 |
| Form/Structure | 20.0 kDa |
Components and Storage
| Formulation | Solution protein |
| Dissolved in sterile PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening. | |
| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice |
Quality
| Purity | > 95%, determined by SDS-PAGE |
| Endotoxin Stability | <0.010 EU per 1 ug of the protein by the LAL method |
| Shipping | Measured in anti- viral assa ys using HeLa humancervical epithelial carcinomacells infected with encephalomyocarditi |
| (EMC) virus.The EC50 for this effect is 1-10 ng/mL. |
SDS-PAGE
Bioactivity
Documents
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