Human CSF3

Bcakground

Granulocyte Colony Stimulating Factor(G-CSF)is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Reference

1. Takano H, et al. (2007) Trends Pharmacol Sci. 28(10): 512-7

2. Klocke R, et al. (2008) Curr Med Chem. 15(10): 968-77.

3. Kang HJ, et al. (2008) Expert Rev Cardiovasc Ther. 6(5): 703-13.

4. Beekman R, et al. (2010) Blood. 115(25): 5131-6.

Specifications

Components and Storage

Quality

SDS-PAGE

Bioactivity

Documents

Human CSF3