HIV-1 p24 ELISA Kit

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Background

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins, which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Features

  • Specification: 96 Test

  • Sensitivity: 0.64 pg/ml (50 μl); 2.62 pg/ml (10 μl)

  • Standard Curve Range: 3.13–200 pg/ml

  • Standard Curve Gradient: 7 Points

  • Number of Incubations: 2

  • Sample Volume: 50 μl / 10 μl

  • Assay Type: Sandwich ELISA

  • Operation Duration: 120 min

Typical data


pg/ml O.D. Average Corrected
0.00 0.0190 0.0200 0.0195  
3.13 0.0300 0.0310 0.0305 0.0110
6.25 0.0520 0.0500 0.0510 0.0315
12.50 0.1150 0.1140 0.1145 0.0950
25.00 0.2920 0.2800 0.2860 0.2665
50.00 0.7590 0.7000 0.7295 0.7100
100.00 1.8220 1.7240 0.7295 1.7535
200.00 3.2790 1.7240 3.1850 3.1655


Precision

  Intra-assay Precision Inter-assay Precision
Sample Number S1 S2 S3 S1 S2 S3
22 22 22 6 6 6
Average(pg/ml) 4.6 18.4 58.9 4.2 19.5 59.7
Standard Deviation 4.6 0.59 4.1 0.21 0.72 3.8
Coefficient of Variation(%) 2.6 3.2 6.9 5.0 3.7 6.4

 

Intra-assay Precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (precision between assays): Three samples of known concentration were tested six times on different plates to assess inter-assay precision.

Spike Recovery

Spike recovery was evaluated by spiking three levels of HIV-1 p24 into serum samples. Unspiked serum was used as a blank in this experiment.

The recovery ranged from 94% to 105%, with an overall mean recovery of 98%.

Storage

Store at 4 °C. Please refer to the protocols for detailed instructions.

Research Use Only

For research purposes only. Not intended for use in diagnostic procedures.

Documents

HIV-1 p24 ELISA Kit

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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