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Sku: FS-Q3001-S
High Sensitivity Probe qPCR Master Mix (Universal)——StarLighter
Description
Ultra-sensitive probe-based qPCR detection places high demands on the reagent: when the starting template is extremely low, such as in single-cell detection or amplification of picogram-level genomic DNA, non-specific amplification is a serious concern and can compromise the reliability of the results.
StarLighter High Sensitivity Probe qPCR Master Mix (Universal) is a probe qPCR master mix based on an ultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase configuration. The optimized buffer reaction system effectively inhibits non-specific amplification and greatly increases the amplification efficiency of qPCR, making it suitable for ultra-sensitive qPCR detection. This product has been verified by animal embryo sex detection and can efficiently amplify genomic DNA with as little as 10 pg of starting template, supporting demanding applications such as single-cell detection and low copy gene detection.
For laboratories performing gene expression analysis or low copy gene detection, this high sensitivity probe qPCR master mix delivers reliable results across a broad range of starting template amounts, from ultra-low concentration to routine samples.
Specification
| Catalog No. | Size | Packaging |
|---|---|---|
| FS-Q3002 | 5 ml | 4 × 1.25 ml per box |
| FS-Q3001-S | 1 ml | 1 tube per box |
Features
- Extremely sensitive, suitable for single-cell detection — in bovine embryo sex identification with a low starting template amount, the Ct value is 2–4 cycles ahead of that of a competitor product.
- Amplifies genomic DNA from as little as 10 pg of starting template — verified by animal embryo sex detection.
- Antibody-blocked hot start — the polymerase is blocked with antibodies, and enzyme activity is restored at above 90 °C for 30 seconds, effectively inhibiting non-specific amplification.
- Ultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase with an optimized buffer reaction system — greatly increases the amplification efficiency of qPCR.
- 5'→3' DNA synthesis with 5'→3' exonuclease activity and no 3'→5' proofreading exonuclease activity, generating 3'-dA protruding ends in the amplified product.
- Fast amplification speed of 1–10 s/kb — compatible with the standard and rapid modes of qPCR instruments.
- Excellent linear range — with human genomic DNA serially diluted from 0.0064 ng to 100 ng, an excellent linear relationship is observed between the log value of the template amount and the Ct value; 5-fold serial dilutions effectively amplify ultra-low concentration templates.
Application
StarLighter High Sensitivity Probe qPCR Master Mix (Universal) is suitable for:
- Gene expression analysis — accurate quantification of target genes.
- Low copy gene detection — reliable detection of ultra-low concentration templates.
- SNP and allele typing — SNP detection and genotyping assays.
- Validation of sequencing and microarray results — confirmatory real-time PCR.
FAQ
What is StarLighter High Sensitivity Probe qPCR Master Mix (Universal)?
It is a probe qPCR master mix based on an ultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase configuration with an optimized buffer reaction system, designed for ultra-sensitive qPCR detection.
How sensitive is this master mix?
It can efficiently amplify genomic DNA with as little as 10 pg of starting template and is extremely sensitive, suitable for single-cell detection.
How does the hot-start mechanism work?
The polymerase is blocked with antibodies. Enzyme activity is restored at above 90 °C for 30 seconds, which effectively inhibits non-specific amplification during reaction setup.
What polymerase is used in this master mix?
It uses an ultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase that catalyzes DNA synthesis in the 5'→3' direction, has 5'→3' exonuclease activity, and has no 3'→5' proofreading exonuclease activity. The amplified product has 3'-dA protruding ends.
What is the amplification speed?
The amplification speed is 1–10 s/kb, and the master mix is compatible with the standard and rapid modes of qPCR instruments.
What is the linear range of this master mix?
Using human genomic DNA serially diluted from 0.0064 ng to 100 ng, there is an excellent linear relationship between the log value of the template amount and the Ct value. The reagent effectively amplifies ultra-low concentration templates in 5-fold serial dilutions (100 ng to 0.0064 ng).
How does it perform in embryo sex detection?
In the identification of the sex of bovine embryos with a low starting template amount, this master mix showed higher sensitivity, with a Ct value 2–4 cycles ahead of that of a competitor product.
What applications is this master mix suitable for?
It is suitable for gene expression analysis, low copy gene detection, SNP and allele typing, and validation of sequencing and microarray results.
What are the available sizes?
The master mix is available as catalog No. FS-Q3002 (5 ml, packaged as 4 × 1.25 ml per box) and FS-Q3001-S (1 ml, packaged as 1 tube per box).
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
