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Full name | HIF1AN rabbit polyclonal antibody |
Alternative names | 50 μl/100 μl |
Reactivity | rabbit polyclonal |
Applications | WB, IHC |
Host | Rabbit |
Clone type | rabbit polyclonal |
Target Background | Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. |
Swissprot No | Q9NWT6 |
Gene Accession | NP_060372 |
WB Predicted band size | Immunohistochemical analysis of paraffin-embedded Human brain tissue or HIF1AN antigen-treated (Neutralization experiment) using P102986(HIF1AN Antibody) at dilution 1/40 |
WB Positive control | P102986-WB-1-P |
WB Recommended dilution | 40 kDa |
IHC predicted cell location | Predicted cell location: Cytoplasm and Nucleus |
IHC positive control | Positive control: Human esophagus cancer |
IHC Recommed dilution | Recommended dilution: 50-300 |
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
P102986-WB-1-P
Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue or HIF1AN antigen-treated (Neutralization experiment) using P102986(HIF1AN Antibody) at dilution 1/40
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