Hamster Primary Gingival Epithelial Cells

Description

Hamster Primary Gingival Epithelial Cells from Cell Biologics are isolated from gingival tissue of hamster. Hamster Primary Gingival Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods.

Hamster Primary Gingival Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Suggested Medium

  Epithelial Cell Medium /w Kit – 500 ml

Catalog No. M6621

Laboratory Applications

Hamster Primary Gingival Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Documents

HM-6202

Cell-Culture-Protocol-CB

Disclaimer

Although Hamster Primary Gingival Epithelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.