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Sku: GMP-TL623-5000
Categories: Magnetic Beads

Background

CD4 is an accessory molecule involved in TCR recognition of MHC class II/peptide complexes. Expressed predominantly on T helper cells, and to a lesser extent on monocytes and dendritic cells, CD4 also serves as the entry receptor for HIV. The CD4 antibody used in NanoSep™ CD4 beads binds most thymocytes and approximately 65% of peripheral blood T cells, enabling targeted enrichment or depletion of CD4⁺ cells.

Description

NanoSep™ CD4 Magnetic Beads are 50 nm magnetic particles conjugated with anti-human CD4 monoclonal antibodies, specifically designed for the positive selection or depletion of CD4⁺ T cells from peripheral blood mononuclear cells (PBMCs). These beads enable efficient and highly specific isolation of CD4⁺ T cells with excellent recovery, purity, and viability. NanoSep™ CD4 beads are compatible with both manual and automated magnetic separation systems, including column-based platforms such as Miltenyi Biotec's MS columns and column-free systems like Applied Cells’ MARS® Bar, making them a flexible and powerful tool for downstream immunology and cell therapy research.

Key Features

  • High cell input capacity: Efficiently processes up to 1×10⁹ PBMCs per run

  • Exceptional performance:

    • Recovery rate: ≥ 95%

    • Purity: ≥ 98% CD4⁺ T cells

    • Viability: ≥ 98% viable post-separation

  • Bead size: 50 nm for optimal labeling and minimal steric hindrance

  • Compatibility: Suitable for both column-based and column-free systems

  • Flexible applications: Supports positive selection or depletion protocols

  • Reactive species: Human

  • Storage: 2–8°C

  • Endotoxin level: < 2 EU/mL

  • Shelf life: 6 months

Applications

NanoSep™ CD4 beads are suitable for isolating CD4⁺ T cells for downstream applications such as:

  • Functional assays (e.g., proliferation, cytotoxicity)

  • Cytokine profiling

  • Co-culture experiments with B cells or dendritic cells

  • Immune monitoring in infectious disease and oncology research

  • T cell expansion and in vitro differentiation protocols

    Compatible Columns and Instruments

    • Column-based separation: MS, LS, and autoMACS® columns (Miltenyi Biotec)

    • Column-free separation: Compatible with MARS® Bar (Applied Cells) and similar platforms

Data Figures

Documents

TL-623_Product Sheet.pdf

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

Terms and Conditions

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