GMO Potatto SEMT5-15 Probe Realtime PCR Kit

Description

GMO Potatto SEMT5-15 Probe Realtime PCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• • Plant Pathogen Surveillance – Rapid qPCR screening of Sweepoviruses in sweet potato (Ipomoea batatas) plants across fields, nurseries, and greenhouses to monitor incidence and spread.

  • Seed/Planting Material Certification – Testing vines, cuttings, tuber sprouts, and in-vitro plantlets to ensure virus-free status before distribution and planting.

  • Breeding & Germplasm Health – Routine indexing of breeding lines and germplasm collections to prevent latent infections and safeguard elite materials.

  • Diagnostics in Plant Clinics – Confirmatory testing from symptomatic and asymptomatic leaf/petiole tissues to support disease diagnosis and management decisions.

  • Epidemiology & Vector Studies – Quantification of viral load for research on whitefly-transmitted begomovirus dynamics, host susceptibility, and control strategies.

  • Post-harvest & Supply Chain QA – Incoming QC for propagation facilities and commercial growers to reduce risk of virus introduction and economic loss.

  Typical sample types: young leaves, petioles, vine tips, tuber sprouts, in-vitro plantlets.
  Matrix compatibility: crude extracts or purified DNA (follow kit protocol for best Ct consistency).

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

GMO Potatto SEMT5-15 Probe Realtime PCR Kit User Manual

MSDS