GFP-Expressing Rabbit Primary Colonic Microvascular Endothelial Cells (0.5x10^6)

Description

In endothelial cell research, visual tracking can be difficult when primary cells are used in co-culture systems, live cell imaging, migration studies, or cell-cell interaction experiments. Researchers often need a primary endothelial cell model that combines tissue relevance with a visible fluorescence signal for easier identification and monitoring during experimental workflows.

GFP-expressing rabbit primary colonic microvascular endothelial cells provide a fluorescence-labeled endothelial model derived from New Zealand White Rabbit colon tissue. The green fluorescent protein signal supports cell tracking and fluorescence imaging when cells are exposed to blue light, while the colonic microvascular endothelial origin supports research related to vascular biology, microvascular systems, and in vitro endothelial models.

These rabbit colonic microvascular endothelial cells are characterized by fluorescence-labeled DiI-Ac-LDL uptake, a functional marker associated with endothelial cells. Cells are supplied cryopreserved and are supported by a standardized primary endothelial cell culture protocol covering recovery, gelatin-coated culture, subculture, and cryopreservation workflows.

Key Features

• GFP-expressing rabbit primary colonic microvascular endothelial cells

• Derived from New Zealand White Rabbit colon tissue

• Green fluorescent protein signal supports endothelial cell tracking and fluorescence imaging

• Characterized by DiI-Ac-LDL uptake as an endothelial functional marker

• Supplied as cryopreserved cells harvested at passage 3

• Expandable for 3–5 passages under recommended culture conditions

• Recommended split ratio: 1:2

• Tested negative for bacteria, yeast, fungi, and mycoplasma

Technical Overview

Applications

• Endothelial Cell Tracking

  GFP expression supports visual tracking of rabbit colonic microvascular endothelial cells in fluorescence-based research workflows.

• Live Cell Fluorescence Imaging

  Useful for experiments requiring identification and observation of GFP-expressing endothelial cells under appropriate fluorescence imaging conditions.

• Colonic Microvascular Research

  Provides a rabbit colon tissue-derived endothelial cell model for studies involving colonic vascular and microvascular biology.

• Vascular Biology Research

  Supports in vitro studies focused on endothelial cell behavior, vascular endothelial models, and microvascular research systems.

• Co-culture Endothelial Models

  GFP labeling can help distinguish endothelial cells from other cell types in mixed culture or co-culture experimental designs.

• Endothelial Cell Characterization Workflows

  DiI-Ac-LDL uptake provides an endothelial functional characterization reference for this cell model.

Cell Culture Workflow

Culture Preparation

• Perform all cell culture procedures in a biosafety cabinet.

• Use aseptic techniques to prevent microbial contamination.

• Sterile filter culture media, supplements, and reagents through a 0.2 μm filter.

• Prewarm only the volume of culture medium needed for immediate use.

Gelatin-Coated Culture Surface

• Coat sterile culture dishes or flasks with gelatin-based coating solution for 2 minutes.

• Aspirate excess coating solution before seeding cells.

• Use gelatin-coated vessels to support endothelial cell attachment and culture workflow consistency.

Cell Recovery from Cryovial

1. Thaw the cryovial quickly in a 37°C water bath until only a few ice pieces remain.

2. Wipe the vial with 70% ethanol after thawing.

3. Transfer cells into a sterile 15 ml conical tube containing pre-warmed culture medium.

4. Centrifuge at 200 × g for 5 minutes to remove residual DMSO.

5. Resuspend the cell pellet in endothelial cell culture medium.

6. Seed cells into a gelatin-coated T25 flask.

7. Culture in a humidified 37°C, 5% CO₂ incubator.

Subculture

• Monitor cells daily for confluence and morphology.

• Recommended split ratio: 1:2.

• Use 0.25% Trypsin-EDTA for cell detachment according to the provided protocol.

• Seed detached cells into fresh gelatin-coated culture vessels.

Cryopreservation

• Recommended freezing medium composition: 10% DMSO, 50% FBS, and 40% culture medium.

• Use controlled cooling at -80°C for 6–12 hours before long-term storage.

• Transfer cryopreserved vials to liquid nitrogen storage.

Storage and Handling

• Transfer frozen cells to liquid nitrogen storage immediately upon receipt.

• Do not store primary cells long-term at -20°C or -80°C.

• Avoid repeated freeze-thaw cycles.

• Handle animal-derived cells with appropriate biosafety precautions.

• For research use only. Not intended for human use, diagnostic procedures, or therapeutic applications.

Quality Control

Documents

N-6203GFP

Cell-Culture-Protocol-CB

Frequently Asked Questions