GFP-Expressing Porcine Primary Mammary Epithelial Cells (0.5x10^6)

Description

Primary mammary epithelial cell models are widely used for studying epithelial biology, tissue development, cellular signaling, and functional responses in mammary systems. However, conventional unlabeled primary cells often require additional staining procedures for visualization, making it difficult to monitor dynamic cellular behaviors such as migration, morphology changes, and cell-cell interactions during live experiments.

GFP-expressing porcine primary mammary epithelial cells (P-6035GFP) provide a fluorescently labeled primary cell model derived from porcine mammary tissue. These cells are isolated from American Landrace pig mammary tissue and genetically modified through GFP lentiviral transduction to generate stable green fluorescent protein expression for real-time observation and tracking.

The GFP-labeled porcine mammary epithelial cells are characterized by immunofluorescence staining using a ZO-1 antibody, confirming epithelial characteristics through tight junction marker expression. The cells are tested negative for bacteria, yeast, fungi, and mycoplasma, providing a reliable research model for mammary epithelial biology, fluorescent imaging, and cell-based assays.

Designed for researchers requiring a physiologically relevant animal primary cell model, these cryopreserved primary mammary epithelial cells support applications including live-cell fluorescence microscopy, epithelial cell culture studies, cell tracking experiments, and comparative mammary biology research.

Features

• GFP-expressing porcine primary mammary epithelial cells derived from porcine mammary tissue
• Stable fluorescent labeling through GFP lentiviral transduction
• Bright green fluorescence for live-cell imaging and fluorescence microscopy
• Characterized by ZO-1 immunofluorescence staining for epithelial identity
• Negative testing for bacteria, yeast, fungi, and mycoplasma
• Cryopreserved primary cells supplied for research applications
• Suitable for mammary epithelial cell culture and cell tracking studies
• Compatible with gelatin-coated culture systems

Specifications

Applications

• Mammary epithelial cell biology research
  Study mammary epithelial cell behavior, morphology, differentiation, and cellular responses using a GFP fluorescent primary cell model.
• Live-cell fluorescence imaging
  Monitor real-time cellular dynamics using GFP fluorescence microscopy without additional fluorescent staining.
• Cell tracking studies
  Track migration, proliferation patterns, and cellular distribution in vitro experiments.
• Epithelial cell culture research
  Evaluate epithelial growth characteristics, cell interactions, and culture conditions.
• Mammary gland biology studies
  Investigate mammary tissue development and epithelial function using a porcine primary cell system.
• Gene expression and cellular signaling studies
  Combine GFP visualization with molecular biology workflows to analyze cellular responses.
• Comparative animal cell research
  Support veterinary, agricultural biotechnology, and animal physiology research.
• Fluorescent cell model development
  Provide a ready-to-use GFP expressing primary cell model for imaging-based experiments.

GFP Fluorescent Imaging System

The integrated GFP labeling system provides a convenient fluorescent cell model for monitoring porcine mammary epithelial cell behavior during live-cell experiments. GFP (Green Fluorescent Protein) produces strong green fluorescence under blue-light excitation, allowing direct visualization using standard fluorescence microscopy systems.

Compared with conventional primary mammary epithelial cells requiring antibody staining or fluorescent labeling before imaging, GFP-expressing cells enable researchers to directly observe dynamic cellular processes including morphology changes, cell distribution, migration behavior, and cell-cell interactions.

• Real-time visualization of mammary epithelial cell morphology
• Fluorescence microscopy-based cell identification and tracking
• Observation of epithelial cell growth patterns and organization
• Monitoring of cellular responses during experimental stimulation
• Compatibility with live-cell imaging workflows and fluorescence analysis platforms

Cell Culture System

GFP-expressing porcine primary mammary epithelial cells are optimized for standard primary epithelial cell culture workflows. Cells are supplied cryopreserved and should be recovered using appropriate aseptic techniques and epithelial cell culture conditions.

Before cell seeding, culture vessels should be coated with a gelatin-based coating solution to support cell attachment and growth. The recommended culture environment is a humidified incubator maintained at 37°C with 5% CO₂.

• Recommended coating: gelatin-based extracellular matrix coating
• Recommended medium: H6621 Complete Epithelial Cell Medium with Kit
• Culture condition: 37°C, 5% CO₂ humidified incubator
• Recommended split ratio: 1:2
• Medium replacement: daily medium changes recommended during expansion
• Recommended handling: sterile biosafety cabinet procedures

According to the manufacturer protocol, cryopreserved cells should be rapidly thawed, washed to remove residual DMSO, and seeded into gelatin-coated culture vessels. Cells are recommended for limited expansion under optimized culture conditions. :contentReference[oaicite:0]{index=0}

Storage & Handling

• Transfer cryopreserved vials to liquid nitrogen storage immediately after arrival.
• Recommended storage temperature: approximately -180°C in liquid nitrogen.
• Do not store cryopreserved cells at -20°C or -80°C for long-term preservation.
• Avoid repeated freeze-thaw cycles to maintain cell viability and GFP expression.
• Use sterile techniques throughout thawing, culture, and experimental procedures.
• Treat all animal-derived cells as potentially biohazardous materials and handle according to institutional guidelines.

Cryopreserved GFP-expressing porcine primary mammary epithelial cells are shipped on dry ice. Upon receipt, cells should be transferred immediately into liquid nitrogen storage until use. :contentReference[oaicite:1]{index=1}

Documents

P-6035GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions