GFP-Expressing Canine Primary Retinal Microvascular Endothelial Cells (0.5x10^6)

Description

In retinal vascular research, understanding endothelial behavior, microvascular formation, and vascular barrier function requires reliable primary cell models that closely represent physiological conditions. However, conventional unlabeled endothelial cells often make it difficult to monitor real-time cellular responses, migration behavior, and vascular remodeling processes during live-cell experiments.

GFP-expressing canine primary retinal microvascular endothelial cells (D-6065GFP) provide a fluorescently labeled primary endothelial cell model derived from canine retinal tissue. These cells are genetically labeled through GFP lentiviral transduction, producing bright green fluorescence that enables direct visualization of endothelial cell morphology, migration, and dynamic cellular changes using fluorescence microscopy.

The cells are isolated from beagle dog retinal microvascular tissue and expanded under optimized endothelial cell culture conditions. Endothelial identity and functionality are characterized by DiI-Ac-LDL uptake assay, a widely used functional marker for endothelial cells. This GFP labeled endothelial cell model is suitable for retinal angiogenesis research, vascular biology studies, endothelial migration assays, permeability analysis, and live-cell fluorescence imaging applications.

Each cryopreserved vial contains 0.5 × 10⁶ cells and is supplied for research use only.

Features

• GFP-expressing canine retinal microvascular endothelial cells for fluorescence-based vascular research

• Derived from primary canine retinal microvascular tissue for physiologically relevant endothelial studies

• Stable GFP fluorescence enables real-time cell tracking and live-cell imaging without additional labeling

• Validated endothelial phenotype through DiI-Ac-LDL uptake assay

• Suitable for retinal angiogenesis, endothelial migration, and vascular remodeling studies

• Compatible with fluorescence microscopy and cell-based imaging workflows

• Cryopreserved primary cell format for flexible experimental scheduling

• Negative testing for bacteria, yeast, fungi, and mycoplasma

Specifications

Applications

• Retinal angiogenesis research
  Study endothelial network formation, angiogenic signaling pathways, and microvascular remodeling processes.

• Retinal vascular biology studies
  Investigate endothelial cell function, vascular development, and microvascular responses under experimental conditions.

• Endothelial migration assays
  Analyze endothelial movement, wound healing responses, and cellular migration behavior using GFP fluorescence tracking.

• Vascular permeability research
  Evaluate endothelial barrier responses and vascular integrity changes in retinal microvascular models.

• Ocular disease research models
  Support studies related to retinal vascular disorders, ocular inflammation, and disease-related endothelial dysfunction.

• Live-cell fluorescence imaging
  Monitor endothelial morphology, cell interaction, and dynamic cellular processes without additional fluorescent staining.

• Drug screening and endothelial response analysis
  Evaluate compound effects on retinal endothelial behavior and vascular-related pathways.

• Cell-based vascular biology assays
  Compatible with endothelial culture workflows including migration, imaging, and functional analysis experiments.

GFP Fluorescent Imaging System

The integrated GFP fluorescence labeling system provides a convenient solution for real-time visualization of canine retinal microvascular endothelial cell behavior. Unlike conventional primary endothelial cells requiring additional fluorescent staining, GFP-expressing cells allow direct observation under fluorescence microscopy after blue-light excitation.

This fluorescent endothelial cell model enables researchers to monitor dynamic biological processes including:

• Endothelial cell morphology changes during culture and experimental stimulation

• Cell migration and directional movement in endothelial migration assays

• Microvascular network formation during angiogenesis studies

• Cell-cell interactions and endothelial remodeling processes

• Real-time tracking of endothelial responses in retinal vascular biology models

Cell Culture System

GFP-expressing canine primary retinal microvascular endothelial cells are optimized for standard endothelial cell culture workflows. The cells are supplied as cryopreserved primary cells and should be cultured under sterile conditions using endothelial growth medium and extracellular matrix-coated culture vessels.

• Recommended coating: Gelatin-based extracellular matrix coating for enhanced endothelial attachment

• Recommended medium: H1168 Complete Endothelial Cell Medium with Kit

• Culture environment: 37°C humidified incubator with 5% CO₂

• Subculture recommendation: Split ratio of 1:2 under recommended culture conditions

• Cell handling: Perform all procedures using aseptic techniques in a biosafety cabinet

For cryopreserved cell recovery, cells should be rapidly thawed, transferred into pre-warmed endothelial growth medium, and plated onto gelatin-coated culture vessels. Medium replacement and daily morphology monitoring are recommended during initial recovery and expansion.

Storage & Handling

• Store cryopreserved cells immediately in liquid nitrogen storage (-180°C) after receiving the shipment.

• Do not store primary endothelial cells at -20°C or -80°C, as these conditions may compromise cell viability.

• Avoid repeated freeze-thaw cycles to maintain GFP expression stability and cell performance.

• Cryopreserved cells are shipped on dry ice and should remain frozen until transfer into long-term storage.

• Protect GFP-expressing cells from unnecessary prolonged exposure to strong light sources.

Quality Control

Documents

D-6065GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions