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GFP-Labeled C57BL/6 Mouse Primary Colon Smooth Muscle Cells for Fluorescence-Based Research
$890.00
Sku: C57-6001GFP
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by September 10, 2026
Description
Fluorescence-based primary cell studies require a clearly defined cell source, stable fluorescent labeling, documented characterization, and a reproducible culture workflow. These GFP-expressing mouse primary colonic smooth muscle cells provide a cryopreserved primary cell model derived from the colon tissue of pathogen-free laboratory C57BL/6 mice.
The cells are isolated from mouse colon tissue and cultured in gelatin-precoated tissue culture flasks using complete smooth muscle cell growth medium. Cells are harvested at passage 2–3 and cryopreserved for frozen delivery. Each vial contains 0.5 × 106 cells per mL.
These GFP-expressing primary cells are generated by transduction with GFP lentiviral particles. Green fluorescent protein produces bright green fluorescence when exposed to blue light, supporting experimental workflows that require fluorescent visualization of primary colonic smooth muscle cells.
Cell identity is characterized by immunofluorescence staining with an antibody against α-smooth muscle actin (α-SMA). The cells are tested and found negative for bacteria, yeast, fungi, and mycoplasma. Under the specified primary cell culture conditions, they can be expanded for approximately 2–3 passages using a recommended split ratio of 1:2.
Repeated freeze-thaw cycles are not recommended because primary cells should be maintained under controlled recovery, expansion, and cryopreservation conditions.
Specifications
| Product Name | GFP-Expressing Mouse Primary Colonic Smooth Muscle Cells |
|---|---|
| Catalog Number | C57-6001GFP |
| Species | Mouse |
| Mouse Strain | C57BL/6 |
| Tissue Source | Colon |
| Cell Type | Primary colonic smooth muscle cells |
| Fluorescent Label | Green fluorescent protein (GFP) |
| GFP Generation Method | Transduction with GFP lentiviral particles |
| Passage at Cryopreservation | Passage 2–3 |
| Cell Quantity | 0.5 × 106 cells per mL |
| Characterization | Immunofluorescence staining for α-smooth muscle actin |
| Expansion Potential | Approximately 2–3 passages under the specified culture conditions |
| Recommended Split Ratio | 1:2 |
| Culture Surface | Gelatin-precoated tissue culture flask or dish |
| Recommended Incubation | 37°C in a humidified incubator with 5% CO2 |
| Quality Testing | Negative for bacteria, yeast, fungi, and mycoplasma |
| Format | Cryopreserved cells |
| Shipping Condition | Frozen shipment on dry ice; live-cell shipment available on request |
| Storage | Liquid nitrogen storage immediately upon receipt |
| Intended Use | For Research Use Only |
Features
- Primary colonic smooth muscle cells isolated from pathogen-free laboratory C57BL/6 mice.
- Stable GFP expression generated through GFP lentiviral particle transduction.
- Bright green fluorescence when exposed to blue light.
- Characterized by immunofluorescence staining for α-smooth muscle actin.
- Cryopreserved at passage 2–3 with 0.5 × 106 cells per mL supplied.
- Expandable for approximately 2–3 passages under the specified culture conditions.
- Recommended for culture in gelatin-precoated vessels with complete smooth muscle cell medium.
- Tested negative for bacteria, yeast, fungi, and mycoplasma.
Applications
GFP-Expressing Mouse Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only.
- Cell-based assays
- Staining procedures
- FACS analysis
Our products are not authorized for human use, or for in vitro diagnostic or therapeutic procedures.
Suggested Medium
| Recommended Medium | M2268 Complete Smooth Muscle Cell Medium with Kit |
|---|---|
| Package Size | 500 mL |
Only the amount of complete culture medium required for immediate use should be prewarmed to 37°C. Repeated warming of complete medium is not recommended.
Culture Information
Culture Vessel Preparation
Coat sterile culture flasks or dishes with a gelatin-based coating solution for approximately 2 minutes. Aspirate the excess coating solution before seeding the cells.
Recovery from Cryopreservation
Thaw the cryovial rapidly in a 37°C water bath for less than 1 minute, or until only a small amount of ice remains. Transfer the cells into prewarmed culture medium and centrifuge at 200 × g for 5 minutes to remove residual cryopreservation medium. Resuspend the cell pellet and seed it into a gelatin-precoated T25 flask.
Incubation
Maintain the cells in a humidified incubator at 37°C with 5% CO2. Following initial seeding, leave the culture undisturbed for approximately 12–16 hours.
Medium Replacement
Replace the medium on the day following recovery and regularly thereafter to remove non-adherent cells and replenish nutrients. Cells should be examined daily for confluence and appropriate morphology.
Subculture
For expansion, wash the adherent cells with calcium- and magnesium-free phosphate-buffered saline and detach them using prewarmed 0.25% Trypsin-EDTA. The recommended split ratio for primary cells is 1:2.
Storage
Cryopreserved cells are shipped frozen on dry ice. Immediately upon arrival, transfer the vial to a liquid nitrogen storage tank until the cells are ready for use.
Do not use a standard −20°C or −80°C freezer for long-term storage of the cryopreserved primary cells. Live-cell shipment may also be available on request.
Documents
Frequently Asked Questions
What are GFP-expressing mouse primary colonic smooth muscle cells?
They are primary smooth muscle cells isolated from the colon tissue of pathogen-free laboratory C57BL/6 mice and modified to express green fluorescent protein through lentiviral particle transduction.
What is the tissue and species source of these primary cells?
The cells are isolated from colon tissue obtained from laboratory C57BL/6 mice.
How are the GFP-expressing primary cells characterized?
The cells are characterized by immunofluorescence staining with an antibody against α-smooth muscle actin.
How many cells are supplied in each cryovial?
Each vial contains 0.5 × 106 cells per mL.
At what passage are the cells cryopreserved?
The cells are harvested and cryopreserved at passage 2–3.
How many passages can these primary colonic smooth muscle cells be expanded?
Under the specified culture conditions, the cells can be expanded for approximately 2–3 passages using a recommended split ratio of 1:2.
What medium is recommended for culturing the cells?
M2268 Complete Smooth Muscle Cell Medium with Kit, supplied in a 500 mL format, is the suggested culture medium.
Do the culture vessels require coating?
Yes. Sterile flasks or dishes should be coated with a gelatin-based coating solution for approximately 2 minutes before the excess solution is aspirated and the cells are seeded.
What incubation conditions are recommended?
The cells should be maintained in a humidified incubator at 37°C with 5% CO2.
How should cryopreserved primary cells be thawed?
Thaw the vial rapidly in a 37°C water bath for less than 1 minute, disinfect the vial with 70% ethanol, transfer the cells into prewarmed culture medium, and centrifuge at 200 × g for 5 minutes before seeding into a gelatin-precoated flask.
Are the cells tested for microbial contamination?
Yes. The supplied cells are reported as negative for bacteria, yeast, fungi, and mycoplasma.
How are the cells shipped and stored?
Cryopreserved cells are shipped on dry ice. Upon arrival, they should be transferred immediately to liquid nitrogen storage. They should not be stored long term in a −20°C or −80°C freezer.
Can the cells be repeatedly frozen and thawed?
No. Repeated freezing and thawing are not recommended.
Are these cells intended for clinical or diagnostic use?
No. The cells are supplied for research use only and are not intended for human use, diagnostic procedures, or therapeutic applications.
Disclaimer
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
For Research Use Only. Not for human use, in vitro diagnostic procedures, or therapeutic applications.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
