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Description
FREY agar is a nutritionally enriched, semi-selective solid medium designed for the cultivation and detection of avian mycoplasmas. The formulation is supplemented with nicotinamide adenine dinucleotide (NAD⁺), a critical coenzyme required for the growth of NAD-dependent species such as Mycoplasma synoviae.
The presence of phenol red as a pH indicator enables visual monitoring of metabolic activity, with a color shift from red to yellow (acidic) or purple (alkaline) indicating the proliferation of mycoplasmal organisms. To selectively inhibit contaminating bacterial flora while preserving mycoplasma viability, penicillin G is incorporated into the medium.
Furthermore, the addition of exogenous lipid supplements from yolk, excluding antibiotics—including cholesterol and long-chain fatty acids—enhances the growth performance of lipid-requiring strains, particularly Mycoplasma pneumoniae ATCC 23714, by supporting membrane biosynthesis and promoting optimal colony formation.
Specifications
| Agency | EP 2.6.7 / USP / JP |
| Quality Level | ISO 9001 |
| Sterility | Sterile |
| - | Aseptically Filled |
| Form | Plate |
| Feature | Ready-to-use; ICR Settle plates |
| packaging | Pack of 10 plates; Box of 10 packs |
| Plate Diam | 60 mm |
| pH | 7.8 (in H2O) |
| Application(s) | Industrial qc |
| Storage Temp | 2-8℃ for 3 months |
| Suitability | Mycoplasma |
Application
FREY agar is recommended for the detection of avian mycoplasmas in pharmaceutical products, particularly in bulk vaccines, cell banks, and virus cultures.
Features
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Ready-to-use convenience — no additional preparation steps are required.
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Broad pharmaceutical applications — suitable for biopharmaceuticals, vaccines, cell cultures, and virus cultures.
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Storage stability — can be stored at 2–8 °C for up to 3 months.
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Rapid detection — a colorimetric chart provides a quick and convenient visual indication of early-stage mycoplasma proliferation.
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Optimized growth performance — the buffered system supports higher colony counts while reducing the risk of mycoplasma cell death.
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Enhanced visualization — a chromogenic reagent selectively stains mycoplasma, improving clarity and specificity of detection under microscopy.
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Improved NAD⁺ stability — to prevent growth inhibition caused by NAD⁺ degradation at later stages, alternative NAD⁺ analogs are co-supplemented in the culture system.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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