Food-Borne Pathogenic Bacteria Multiplex Probe Method qPCR Kit

Description

Food-Borne Pathogenic Bacteria Multiplex Probe Method qPCR Kit is designed for the quantitative detection of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.

Key Features

One-tube qPCR format minimizes carry-over contamination risk

• High sensitivity: analytical LOD as low as 100 copies/reaction

• Excellent specificity: no cross-reactivity with DNA from other pathogens

• Includes amplification positive control for result verification

• Instrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc.

• Suitable for both qualitative and quantitative applications

• For quantitative use: the linear range covers a 100,000-fold concentration difference.

• Sufficient for 50 reactions (20 μL each)

• For Research Use Only (RUO)

Kit Components

Application Areas

• Aquaculture Health Management & Pathogen Surveillance: Rapid multiplex qPCR screening of Vibrio spp., L. monocytogenes and other food-borne bacteria in abalone, shrimp and finfish farms to prevent disease outbreaks and optimize biosecurity.
• Seafood Processing Quality Control & Regulatory Compliance: Sensitive detection of multiple marine pathogen markers in raw and processed seafood to support HACCP workflows, meet FDA/EU standards and ensure product safety.
• Marine Ecosystem Pathogen Monitoring & Research: Quantitative analysis of water, sediment and biota samples for temporal and spatial mapping of pathogen dynamics, aiding environmental impact studies and epidemiological investigations.

Storage & Shipping

• Store at - 20 °C

• The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.
  

Documents

Food-Borne Pathogenic Bacteria Multiplex Probe Method qPCR Kit User Manual

MSDS