Diabetic Mouse Coronary Artery Endothelial Cells

Description

Diabetic Mouse Primary Coronary Artery Endothelial Cells from Cell Biologics are isolated from coronary artery of pathogen-free Diabetic (db/db) mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Diabetic Mouse Primary Coronary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (DIABETIC44, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R&D System) or CD31/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Suggested Medium

M1168 Complete Endothelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

MD-6093

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.