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$470.00
Sku: BF-38651768
Availability:
AVAILABLE
In stock & estimated to ship in 7-14 days by October 07, 2026
Description
Cyclospora cayetanensis Probe qPCR Kit is a research-use-only (RUO), probe-based real-time PCR assay for the sensitive and specific detection of Cyclospora cayetanensis nucleic acid — the coccidian parasite associated with cyclosporiasis outbreaks linked to contaminated fresh produce.
Purified DNA is amplified directly using a sequence-specific primer–probe set with TaqMan probe chemistry. During amplification, fluorescence generated by probe cleavage is monitored in real time (FAM channel), enabling detection and comparative analysis of target nucleic acid in a one-step PCR workflow.
The kit is suited to food-safety and method-development research — for example, evaluating C. cayetanensis detection in produce wash-off or environmental samples against established reference approaches such as the FDA Bacteriological Analytical Manual (BAM) Chapter 19b real-time PCR method. All components are optimized for research use; the kit is not intended for diagnostic or therapeutic applications.
Note: Supplied without internal control. An internal-control version can be customized based on your assay requirements.
This product was previously listed under catalog number 15-71400 and is now updated to BF-38651768 for improved product management.
Key Features
- One-step PCR workflow to reduce handling steps and minimize contamination risk.
- TaqMan probe chemistry — FAM channel detection, compatible with virtually all standard real-time PCR platforms.
- High sensitivity and specificity based on target-specific primer–probe design.
- Includes a non-infectious plasmid-based positive control (1E7 copies/µL) for assay verification.
- Compatible with commonly used DNA purification methods, including column- and magnetic-bead-based extraction.
- Suitable for research applications including assay development and method evaluation.
- Broad dynamic range under laboratory conditions.
- For research use only. Not intended for diagnostic or therapeutic use.
Assay Specifications
| Parameter | Specification |
| Target | Cyclospora cayetanensis (target-specific primer–probe set; assay details per user manual) |
| Detection chemistry | TaqMan probe, FAM channel |
| Reaction volume | 20 µL (10 µL 2× Probe qPCR Master Mix + 3 µL Primer-Probe Mix + 7 µL template) |
| Reactions per kit | 50 |
| Cycling conditions | 95 °C 5 min; 45 cycles × (95 °C 15 s / 60 °C 30 s / 72 °C 1 min, FAM acquisition) |
| Positive control | 1E7 copies/µL, non-infectious plasmid |
| Storage & stability | Store at −20 °C; stable for at least 12 months under recommended conditions |
Analytical performance data (e.g., linear range, sensitivity) are available on request — see the validation-data inquiry below.
Workflow
- Extract — Prepare purified DNA using column- or magnetic-bead-based extraction methods.
- Set up — Reconstitute the lyophilized primer–probe powder, then prepare 20 µL reactions on ice.
- Run — Perform real-time PCR on a standard instrument (FAM channel); review Ct values and control performance as described in the user manual.
Full step-by-step instructions: User Manual (PDF)
Kit Components
| Component Name | Description | Size | Vial | Lid Color |
| 2×Probe qPCR Master Mix | Contains optimized buffer and enzyme mix | 500 µL | 0.5 mL Vial | Blue |
| Primer-Probe Powder | Lyophilized target-specific primer and probe set | 50 T | 1.5 mL Vial | Brown |
| Positive Control (1E7 copies/µL) | Non-infectious plasmid-based control | 0.5 mL | 0.5 mL Vial | Yellow |
| Nuclease-free water / Dilution Buffer | For reaction setup | 1 mL | 1.5 mL Vial | Green |
| Manual | Instruction booklet | 1 Set | NA | NA |
Storage & Shipping
- Store at −20 °C. The kit is stable for at least 12 months under recommended storage conditions; avoid repeated freeze–thaw cycles.
- Shipped on dry ice to maintain low-temperature conditions and ensure product integrity in transit.
- Kits are produced to order: standard production takes 7–14 days before dispatch. Expedited production is available on request (see FAQs below).
FAQ
No — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at contact@biofargo.com.
Unless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at contact@biofargo.com.
Validation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at contact@biofargo.com.
In clinical and outbreak settings, C. cayetanensis is typically identified by microscopy (e.g., modified acid-fast staining) and confirmed by molecular methods. For food-safety research, the FDA Bacteriological Analytical Manual (BAM) Chapter 19b describes a real-time PCR method for detecting C. cayetanensis in fresh produce.
This kit applies the same detection principle in a probe-based real-time PCR format (FAM channel, TaqMan chemistry), suitable for research laboratories running purified-DNA workflows. For research use only; not for diagnostic use. See the probe qPCR kit collection for related assays.
C. cayetanensis outbreaks are most frequently linked to imported fresh produce, including berries (e.g., raspberries and blackberries), fresh herbs (basil, cilantro), and leafy greens or salad mixes. For food-safety research, produce wash-off or rinse samples can be processed for nucleic acid detection with this kit.
Cyclospora oocysts are sensitive to adequate heat, and thorough cooking of produce is a standard food-safety control measure. Note that typical refrigeration or freezing is not a reliable way to inactivate oocysts — which is why PCR-based detection methods are used in food-safety research to verify produce and wash water.
Standard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.
Yes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at contact@biofargo.com.
Our kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.
Yes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:
- 5% OFF (Qty. 5–9 kits)
- 10% OFF (Qty. 10–14 kits)
- 25% OFF (Qty. 15–29 kits)
- 35% OFF (Qty. 30+ kits)
For more information about discounted pricing, please contact us at contact@biofargo.com.
Yes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.
No. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20 °C for best performance.
No. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.
Documents
Cyclospora cayetanensis Probe qPCR Kit User Manual
Related Products & Support
Complete your detection workflow with DNA extraction kits and qPCR consumables — see the full probe qPCR kit collection for the complete product range.
Need performance data or a custom quote? Contact our team: Request Validation Data
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
