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$470.00
Sku: BF-56560001
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by September 15, 2026
Description
The Crithidia fasciculata Probe qPCR Kit is designed for the qualitative detection of Crithidia fasciculata DNA using probe-based real-time PCR. Purified DNA is amplified with target-specific primers and a fluorescent probe, enabling real-time monitoring of the amplification reaction.
This kit provides researchers with a targeted molecular method for detecting C. fasciculata DNA in prepared research samples. It may support laboratory studies involving kinetoplastid biology, cultured organisms, kinetoplast DNA, and insect–protozoan interactions.
Note: This product is supplied without an internal control. Sample compatibility and analytical performance depend on the validated nucleic acid extraction and testing workflow.
Specification
| Product Name | Crithidia fasciculata Probe qPCR Kit |
|---|---|
| Target Organism | Crithidia fasciculata Léger |
| Target Material | Extracted DNA |
| Detection Technology | Probe-based real-time PCR |
| Detection Type | Qualitative detection |
| Internal Control | Not included |
| NCBI Taxonomy ID | 5656 |
| Intended Use | Laboratory research use only |
Features
- Designed for qualitative detection of Crithidia fasciculata DNA.
- Uses target-specific primer and probe chemistry.
- Enables real-time fluorescence monitoring during amplification.
- Suitable for purified DNA prepared from research samples.
- Includes a non-infectious plasmid-based positive control for assay verification.
- Intended exclusively for laboratory research applications.
Kit Components
| Component | Description | Size | Vial | Lid Color |
|---|---|---|---|---|
| 2× Probe qPCR Master Mix | Optimized buffer and enzyme mixture | 500 µL | 0.5 mL vial | Blue |
| Primer-Probe Powder | Lyophilized target-specific primer and probe set | 50 reactions | 1.5 mL vial | Brown |
| Positive Control | Non-infectious plasmid-based control | 0.5 mL | 0.5 mL vial | Yellow |
| Nuclease-Free Water | For reaction preparation and dilution | 1 mL | 1.5 mL vial | Green |
| User Manual | Product instructions | 1 set | — | — |
Application
- Qualitative detection of Crithidia fasciculata DNA.
- Molecular confirmation of laboratory cultures.
- Screening in insect–protozoan interaction studies.
- Kinetoplast and trypanosomatid molecular biology research.
Use extracted DNA of suitable purity. The applicable sample types should be determined according to the validated extraction method and laboratory workflow.
Storage & Shipping
- Store the kit at −20°C.
- Ship under dry-ice conditions.
- Avoid repeated freeze–thaw cycles.
- Protect fluorescent probe components from prolonged light exposure.
Frequently Asked Questions
The kit is designed for the qualitative detection of Crithidia fasciculata DNA using probe-based real-time PCR.
No. The standard kit is supplied without an internal control. An internal-control configuration may be requested separately.
The kit is intended for purified DNA prepared from research samples. Compatibility depends on the selected nucleic acid extraction method and validated workflow.
No. This kit is intended strictly for laboratory research use and is not intended for clinical diagnostic or therapeutic applications.
For Research Use Only. Not for use in diagnostic procedures.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
