Broad Range 4-Color Prestained Protein Marker (9 bands 10-270 kDa)

Description

This 4-color prestained protein ladder contains 9 protein bands ranging from 10 to 270 kDa in Tris-Glycine buffer, covering a broad molecular weight range suitable for routine SDS-PAGE and Western blot applications. It enables real-time monitoring of protein separation, accurate estimation of target protein molecular weight, and evaluation of transfer efficiency. In addition, the 10 kDa, 25 kDa, 110 kDa, and 130 kDa bands are labeled with fluorescent dyes, making the ladder compatible with near-infrared fluorescence imaging systems.

The Broad Range Prestained Protein Marker is ideal for:

• Monitoring protein separation during SDS-PAGE

• Verifying Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon)

• Estimating molecular weights of target proteins

Features

• Rich Color Differentiation: Multicolor bands enhance visibility during electrophoresis and membrane transfer, making it easy to identify and distinguish bands at a glance.

• Superior Accuracy & Separation: Sharp and evenly resolved bands enable precise protein molecular weight estimation, delivering performance comparable to premium imported markers.

• High Stability: Maintains integrity without noticeable changes when stored at 37°C for up to two weeks.

• Bright & Concentrated Bands: Strong signal intensity with high protein concentration; only 3 µL loading achieves the same performance as 5 µL of competitor products.

• Multicolor Visualization: Five distinct band colors allow for easier identification and orientation during gel runs and imaging.

• Low Cross-reactivity: Protein tags are removed to minimize non-specific binding during immunodetection.

Applications

• SDS-PAGE Protein Electrophoresis: Used for monitoring protein separation during SDS-PAGE and providing a visible molecular weight reference.

• Western Blot Analysis: Used as a protein ladder for Western blot experiments to estimate target protein molecular weight.

• Western Blot Transfer Verification: Helps evaluate transfer efficiency on nitrocellulose, PVDF, or nylon membranes.

• Near-Infrared Fluorescence Imaging: Suitable for imaging systems using near-infrared fluorescence detection due to fluorescently labeled reference bands.

• Protein Molecular Weight Estimation: Provides a reference standard for estimating the molecular weight of target proteins.

Storage Conditions

• Store at –20°C for long-term storage.

• For frequent use, it can be stored at 4°C for up to 3 months.

• Avoid repeated freeze-thaw cycles.

Competitive Data

Frequently Asked Questions