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Specification
| Quality Level | ISO 9001 |
| Sterility | Sterile |
| Form | Liquid |
| Feature | Ready-to-use for cells; 10ml for 1L Mycoplasma broth |
| Packaging | 1 bottle |
| Bottle Filling Volume | 100 mL |
| PH | 7.4 (in H2O) |
| Storage Temp | 2-8℃ for 6 months；-20℃/ -80℃ for 10 months |
| Suitability | Mycoplasma、cells |
Application
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General cell culture:
Supports the growth and maintenance of a variety of human and animal cell lines, including primary cells, fibroblasts, and tumor cell cultures.
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Tissue and organ culture:
Due to its inclusion of nucleosides and coenzymes (such as NAD, NADP, and Coenzyme A), CMRL-1066 is suitable for short-term maintenance of mammalian tissue fragments or organ explants in vitro.
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Low-serum or serum-free systems:
The presence of ascorbic acid, glutathione, cholesterol, and Tween 80 helps maintain membrane stability and cellular vitality under low-serum conditions. Hence, it is frequently used in the development of serum-free or chemically defined culture systems.
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Host–pathogen co-culture studies:
As a nutritionally rich basal medium, CMRL-1066 is often employed for the co-culture of cell-dependent microorganisms such as Mycoplasma, Rickettsia, or certain viruses.
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Cell recovery and pre-freezing maintenance:
With multiple antioxidant and metabolic support components (e.g., glucose, niacinamide, FAD, glutathione), it is also used for short-term recovery culture prior to cryopreservation or thawing.
Features
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Enhanced Lipid Supplementation:
Incorporation of soluble lipid components improves cellular and mycoplasma metabolism, optimizes membrane composition, and enhances stress resistance—resulting in superior culture performance.
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Stable Physiological Buffering:
Formulated with 20 mM HEPES, maintaining pH precisely at 7.4 to ensure consistent environmental stability throughout incubation and handling.
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Serum Protein Enrichment:
Addition of defined serum proteins enhances culture stability and reinforces overall environmental consistency for reproducible results.
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Simplified, Indicator-Free System:
Sodium bicarbonate and phenol red have been removed to reduce variability and eliminate potential optical or chemical interferences during sensitive assays.
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Optimized Amino Acid Profile:
L-glutamine and L-cysteine are excluded to minimize degradation-related byproducts and maintain long-term formulation stability.
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Light-Protective Packaging:
Supplied in amber bottles to preserve the integrity of light-sensitive components and ensure sustained biochemical activity during storage.
| Inorganic Salts |
| Calcium chloride dihydrate |
| Magnesium sulphate anhydrous |
| Potassium chloride |
| Sodium acetate anhydrous |
| Sodium chloride |
| Sodium dihydrogen phosphate anhydrous |
| Amino Acids |
| Glycine |
| L-Alanine |
| L-Arginine |
| L-Aspartic acid |
| L-Cystine |
| L-Glutamic acid |
| L-Histidine hydrochloride monohydrate |
| L-Isoleucine |
| L-Leucine |
| L-Lysine hydrochloride |
| L-Methionine |
| L-Phenylalanine |
| L-Proline |
| L-Serine |
| L-Threonine |
| L-Tryptophan |
| L-Tyrosine disodium salt dihydrate |
| L-Valine |
| Trans-4-Hydroxy-L-Proline |
| Vitamins & Cofactors |
| Choline chloride |
| D-Biotin |
| D-Pantothenate (hemicalcium) |
| Folic acid |
| L-Ascorbic acid sodium salt |
| Nicotinamide |
| Nicotinic acid |
| Pyridoxal hydrochloride |
| Pyridoxine hydrochloride |
| Riboflavin |
| Thiamine hydrochloride |
| myo-Inositol |
| p-Amino benzoic acid (PABA) |
| Nucleosides & Derivatives |
| 2′-Deoxyadenosine |
| 2′-Deoxycytidine hydrochloride |
| 2′-Deoxyguanosine |
| 5-Methyldeoxycytidine |
| Cholesterol |
| Cocarboxylase |
| Coenzyme A sodium salt |
| D-Glucose |
| D-Glucuronic acid sodium salt |
| FAD disodium salt |
| Glutathione (reduced) |
| Thymidine |
| Uridine-5-Triphosphate sodium salt |
| β-NAD |
| β-NADP sodium salt Lecithin |
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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