0 item(s)
View cart
You have no items in your shopping cart.
Description
Molecular Formula: C32H58N2O7S
Molecular Weight: 614.9
CHAPS is a standard reagent has been found suitable for use as a solubilizing agent in isoelectric focusing applications. It is tested to meet low metal content specifications.
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent for solubilizing membrane proteins and breaking protein-protein interactions. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis, especially for nondenaturing (without urea) IEF.
CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (w/v) are typically used in an IEF gel. A commonly used IEF sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT), and 40 mM Tris. Furthermore, its small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allow it to be removed from samples by dialysis
Specifications
| Grade | Ultra Pure Grade |
| Formula | C32H58N2SO7 |
| MW | 614.89 |
| CAS | 75621-03-3 |
| Appearance | White to off-white powder |
| Assay | ≥98% |
| Loss on drying | ≤4.0% |
| TLC | One spot |
| Solubility (10% aq.) | Clear, colorless to slightly yellow solution |
| Storage | 2~8℃ |
Application
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Warning
Toxicity: Harmful (C)
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
Publication
Chen, Y., et al. 1992. Biochemistry 31, 2415.
Ofri, D., et al. 1992. J. Neurochem. 58, 628.
Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823.
Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA77, 4623.
Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry31, 576.
Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA77, 6368.
Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA77, 4623.
Documents
INFO.
Disclaimer: For laboratory research use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
No data
