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Extract cfDNA from up to 50 mL urine in a single prep — ideal for ultra-low abundance circulating DNA
Description
The Human Urine cfDNA Extraction Kit (Cat. CF-DNA-301) is designed for high-efficiency isolation of circulating cell-free DNA (cfDNA) from up to 50 mL of urine in a single preparation. The kit utilizes an innovative double-layer column technology that maximizes cfDNA recovery while eliminating genomic DNA contamination from lysed white blood cells.
The eluted cfDNA is highly pure and immediately ready for downstream molecular applications, including next-generation sequencing (NGS), qPCR, digital PCR, and methylation analysis.
This kit is particularly suited for applications requiring large input volumes, such as early-stage cancer detection or liquid biopsy studies where cfDNA abundance is low.
Performance Specifications
| Parameter | Specification |
| Sample volume | Up to 50 mL urine |
| cfDNA fragment size | 80–500 bp |
| Recovery rate | ≥90% |
| Yield | >20 μg total cfDNA |
| Purity | Direct-use for NGS / PCR |
| Genomic DNA contamination | None detectable (WBC gDNA retained on membrane) |
| Format | 50 Preps |
Applications
This high-capacity urine cfDNA extraction kit is optimized for:
-
Early cancer detection & minimal residual disease (MRD)
(large input volume improves sensitivity for low-abundance markers) -
Liquid biopsy & non-invasive genomic profiling
-
Urothelial carcinoma screening
-
Prenatal screening (urine-based cfDNA research)
-
Transplant rejection monitoring
-
Infectious disease monitoring via urinary cfDNA
-
Epigenetic and methylation assays
-
Biomarker discovery from low-input urine samples
Large-volume capability significantly enhances assay sensitivity, making the CF-DNA-301 especially suitable for early-stage or low-abundance cfDNA studies where typical 1–5 mL plasma inputs are insufficient.
Sample Volumes
| Kit Variant | Sample Volume |
| #301 | Up to 50ml of Urine |
| #302 | Up to 5ml of Plasma |
| #303 | Up to 0.7ml of Plasma |
Storage
Store at RT (20-25C).
Related Products
(Click Here) #302 cf-DNA Extraction Kit
(Click Here) #303 cf-DNA Extraction Kit
Product Manual
This product is intended for research use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

