T25 Cell Culture Flasks with Vented Cap NonTreated Sterile Biofargo

A T25 cell culture flask is designed for the growth, maintenance, and expansion of suspension mammalian cells. With a 25 cm² growth surface, it is commonly used for routine suspension cell culture, cell line maintenance, protein expression, and preparation of cells for downstream assays.

Non-Treated refers to a cell culture flask without tissue culture surface treatment. The untreated hydrophobic surface is suitable for suspension cell cultures or applications where cell attachment is not required.

A T25 flask provides a growth surface area of 25 cm² and a maximum volume of 50 mL. This size is commonly used for routine suspension cell culture and offers convenient handling in the laboratory.

Although the maximum volume is 50 mL, most cell culture protocols recommend using approximately 5–10 mL of culture medium in a T25 flask. The optimal volume depends on the cell type and specific experimental requirements.

A vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.

The 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.

Yes. The non-treated surface is suitable for suspension cell lines that do not require attachment for growth. It is commonly used for suspension mammalian cells and other applications where cell adhesion is unnecessary.

Yes. Biofargo T25 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.

Yes. Biofargo T25 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.

The flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell cultures. The cap is made of HDPE.

Yes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.

T25 non-treated cell culture flasks are widely used for suspension cell culture, hybridoma culture, protein expression, bioprocess development, cell maintenance, and general life science research where cell attachment is not required.