CD1 Mouse Primary Splenic Cells

Description

CD1 Mouse Primary Spleen Cells from Cell Biologics are isolated from the spleen tissue of pathogen-free laboratory mice. CD1 Mouse Primary Spleen Cells are mixed population of CD1 Mouse spleen cells grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10⁶ cells per ml and is delivered frozen.

Product Testing

CD1 Mouse Primary Spleen Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Suggested Medium

 Cell Culture Medium /w Kit – 500 ml

Catalog No. M5567

Laboratory Applications

CD1 Mouse Primary Spleen Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent flow cytometry. 

Storage

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Documents

Cell-Culture-Protocol-CB

Disclaimer

Although CD1 Mouse Primary Spleen Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution, since no test procedure can completely guarantee the absence of infectious agents.