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Description
Recovering small extracellular vesicles from cell culture supernatant, serum, or plasma can involve complicated sample preparation, sample concentration, or expensive equipment. CATAROSEV® Small EV Isolation Kit provides a membrane-based solution for separating and recovering small EVs without complicated sample concentration or ultracentrifugation equipment.
CATAROSEV® uses a precision filtration membrane with ion-exchange functionality and size-separation capability. The small EV isolation workflow uses the size and surface charge of extracellular vesicles to complete adsorption, washing, and elution. Small EVs adsorbed to the membrane are recovered with a high-salt elution buffer.
The procedure can be completed in approximately 30 minutes. According to the stated product information, the kit efficiently recovers intact particles within the 50–150 nm range. The product has been used to recover small EVs from samples including cell culture supernatant, serum, plasma, fruit juice, bacterial samples, tomato fruit, and peripheral blood.
Catalog number CTS-001 contains five single-use syringe filters and five three-way stopcocks. The required salt concentration adjustment, equilibration/washing, and elution buffers are not included.
Specification
| Product Name | CATAROSEV® Small EV Isolation Kit |
|---|---|
| Catalog Number | CTS-001 |
| Product Type | Small EV and Exosome Isolation Kit |
| Purpose | Isolation and recovery of small extracellular vesicles |
| Isolation Principle | Ion exchange and size separation |
| Membrane Pore Size | Approximately 200 nm |
| Target Particle Range | 50–150 nm |
| Workflow | Adsorption, washing, and elution |
| Processing Time | Approximately 30 minutes |
| Recommended Cell Culture Supernatant Input | Approximately 10–15 mL |
| Recommended Serum Input | Approximately 100–200 μL |
| Recommended Plasma Input | Approximately 100–200 μL |
| Elution Buffer Volume | 2 mL |
| Approximate Eluate Salt Concentration | 0.25 M |
| Pack Size | 5 uses |
| Storage | Refrigerated storage |
| Temporary Room-Temperature Storage | Up to 3 months |
| Reuse | Single use only |
| Intended Use | Research use only |
Kit Components
| Component | Quantity |
|---|---|
| Syringe filter with ion-exchange membrane | 5 sets |
| Three-way stopcock | 5 sets |
Required but Not Included
- Sample containing extracellular vesicles
- Four 20 mL syringes per procedure
- One 5 mL collection tube per procedure
- Benchtop centrifuge and centrifuge tubes
- Protective equipment, including protective glasses and gloves
- Salt concentration adjustment buffer
- Equilibration and washing buffer
- Elution buffer
A low-protein-binding collection tube is recommended. The three required buffers can be prepared according to the instructions or obtained separately as Buffer Set for CATAROSEV®, catalog number CTS-011.
Features
- Membrane-based small EV isolation using particle size and surface charge.
- Precision filtration membrane with ion-exchange and size-separation functions.
- Approximately 30-minute small EV recovery procedure.
- No complicated sample concentration is required.
- No expensive ultracentrifugation equipment is required.
- Three-step adsorption, washing, and elution workflow.
- Efficient recovery of intact particles within the 50–150 nm range.
- Applicable to cell culture supernatant, serum, and plasma sample preparation.
- Five single-use isolation sets per CTS-001 kit.
Isolation Principle
- Adsorption: The prepared sample is introduced into the CATAROSEV® membrane, where small EVs adsorb through their surface charge.
- Washing: Equilibration and washing buffer is introduced to remove uncharged contaminants.
- Elution: A high-salt elution buffer is used to recover the small EVs adsorbed within the membrane.
Application
CATAROSEV® is intended for the isolation and recovery of small extracellular vesicles from research samples. The stated sample formats include cell culture supernatant, serum, and plasma.
- Small EV isolation from cell culture supernatant
- Small EV isolation from serum
- Small EV isolation from plasma
- Extracellular vesicle particle-size analysis
- Western blot analysis of recovered extracellular vesicles
- MALDI-TOF MS analysis of extracellular vesicles recovered from peripheral blood
Additional examples presented in the product information include extracellular vesicle recovery from bacterial samples and tomato fruit. Sample suitability may vary according to sample composition and preparation conditions.
FAQ
What is the CATAROSEV® Small EV Isolation Kit used for?
CATAROSEV® is used to isolate and recover small extracellular vesicles from research samples using a precision filtration membrane with ion-exchange and size-separation functions.
How does this membrane-based exosome isolation kit work?
The kit separates small EVs according to particle size and surface charge. The workflow consists of adsorption to the membrane, washing to remove contaminants, and high-salt elution to recover the adsorbed small EVs.
Does CATAROSEV® require ultracentrifugation?
No expensive ultracentrifugation equipment is required for the stated CATAROSEV® isolation workflow.
Is sample concentration required before small EV isolation?
The product does not require complicated pretreatment such as sample concentration. Sample-specific preparation is still required according to the instructions.
How long does the small EV recovery procedure take?
The stated processing time is approximately 30 minutes.
What particle size range can be recovered?
CATAROSEV® is designed to efficiently recover intact particles within the 50–150 nm range.
What is the approximate membrane pore size?
The precision filtration membrane has an approximate pore size of 200 nm.
Can the kit isolate small EVs from cell culture supernatant?
Yes. The recommended cell culture supernatant input is approximately 10–15 mL.
What preparation is recommended for cell culture supernatant?
If coarse particles may be present, prefiltration with a 0.45 μm filter is recommended. The sample should be adjusted to a salt concentration of approximately 0.1 M using salt concentration adjustment buffer.
Can CATAROSEV® be used with serum or plasma?
Yes. The recommended serum or plasma input is approximately 100–200 μL.
How should serum or plasma be prepared?
Serum or plasma should be centrifuged at 10,000 × g for 5 minutes at room temperature. The recovered supernatant should then be diluted at least 50-fold with equilibration and washing buffer.
What components are included with catalog number CTS-001?
CTS-001 contains five syringe filters with ion-exchange membranes and five three-way stopcocks.
Are the required buffers included in CTS-001?
No. Salt concentration adjustment buffer, equilibration and washing buffer, and elution buffer are not included.
Is a separate buffer set available?
Yes. Buffer Set for CATAROSEV®, catalog number CTS-011, contains 100 mL each of salt concentration adjustment buffer, equilibration and washing buffer, and elution buffer.
How much washing buffer is used per procedure?
The membrane equilibration step uses 5 mL of equilibration and washing buffer. The washing step uses an additional 10 mL.
How much elution buffer is used?
The elution step uses 2 mL of elution buffer.
What is the approximate salt concentration of the recovered eluate?
The recovered eluate has an approximate salt concentration of 0.25 M. The salt concentration can be adjusted when required for subsequent experiments.
Can a CATAROSEV® syringe filter be reused?
No. Each filter is intended for a single use and should not be washed or reused.
How should CATAROSEV® be stored?
The kit should be stored under refrigerated conditions. Storage at room temperature is possible for up to three months.
What precautions should be taken during operation?
Air should not be allowed to enter the syringe filter because trapped air may prevent an appropriate recovery volume. Samples should also be handled using protective equipment and according to the applicable laboratory safety requirements.
Is CATAROSEV® intended for diagnostic or therapeutic use?
No. CATAROSEV® is a research-use reagent. It must not be used as food or as a cosmetic product.
