Carboxypeptidase B

Description

Working with a lyophilized enzyme usually raises three immediate questions in the lab: how do I reconstitute it, how do I store it, and how much do I use for my reaction? This section answers all three using the published product specifications.

Carboxypeptidase B (Catalog #BF-1210200) is supplied as a freeze-dried powder sourced from porcine pancreas. The freeze-dried format avoids the instability that often accompanies ready-to-use liquid enzyme preparations, and it lets you weigh out exactly the amount you need.

Reconstitution is straightforward: dissolve the freeze-dried powder in distilled water or a dilute buffer. After reconstitution, store at -20°C (-4°F) to keep activity stable.

Two reported values let you plan your experiment precisely: an activity of approximately 50 U/mg protein and a protein content of approximately 80%, as determined by the biuret method. Together these two figures give you a direct basis for calculating enzyme-to-substrate ratios.

Specification

Features

• Freeze-dried powder form — convenient to weigh, aliquot, and store.
• Sourced from porcine pancreas.
• Soluble in distilled water or a dilute buffer — simple reconstitution.
• Store at -20°C (-4°F) for stability.
• Transparent activity specification: approximately 50 U/mg protein.
• Transparent protein content: approximately 80%, by the biuret method.
• Unit price $3.00 per mg.

Application

Supplied as a freeze-dried powder from porcine pancreas, this Carboxypeptidase B (Catalog #BF-1210200) is prepared for laboratory and biochemical research use. Its specifications — activity of approximately 50 U/mg protein and protein content of approximately 80% (biuret) — are reported so that experiments can be set up with consistent, reproducible enzyme amounts.

Frequently Asked Questions