0 item(s)
View cart
You have no items in your shopping cart.
Description
CAPS is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 9.7 – 11.1 and commonly used to study enzymatic processes above physiological pH. CAPS is an excellent buffer for the transfer of high molecular weight proteins to nitrocellulose membranes after SDS-PAGE. It is also used in protein sequencing. CAPS displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in solutions containing metal ions. CAPS is suitable for use with the bicinchoninic acid (BCA) assay.
Specifications
| Item | Value |
| Chemical Name | 3-(Cyclohexylamino)-1-Propanesulfinic Acid |
| Appearance | White Crystal |
| CAS No. | 1135-40-6 |
| Molecular Formula | C9H19NO3S |
| Molecular Weight | 221.3 |
| Grade | Biochemical Grade |
| Purity | ≥99.0% |
| Water Solubility | Colorless, Clear, Transparent |
| Heavy Metals | ≤3ppm |
| Moisture | ≤0.5% |
| pH (1% Aqueous Solution at 25℃) | 4.8-6.0 |
| UV Absorbance at 260nm | ≤0.020abs unit |
| UV Absorbance at 280nm | ≤0.038abs unit |
| Storage / Handling | Store at Room Temperature. |
Features
-
High-Purity Grade: ≥ 99.0% biochemical grade ensures minimal impurities, suitable for protein sequencing and sensitive assays.
-
Optimal Alkaline Buffering Range: Effective buffering between pH 9.7–11.1, supporting enzymatic reactions and protein analyses.
-
Low UV Absorbance & Low Interference: Minimal absorbance at 260 nm / 280 nm reduces background interference in nucleic acid or protein assays.
-
Non-Coordinating with Metal Ions: Weak or negligible complexation with metal ions, preserving enzyme or protein activity.
-
Excellent Solubility and Stability: Water-soluble, clear solution. Solid powder stable at room temperature and easy to handle.
Applications
-
Protein electrophoresis & Western blot transfers: Suitable for SDS-PAGE and transfer of high molecular weight proteins to nitrocellulose or PVDF membranes.
-
Protein sequencing & sample preparation: Ideal for N-terminal protein sequencing without buffer interference.
-
Enzymatic assays under alkaline conditions: Supports enzyme kinetics and assays requiring pH above physiological range.
-
Protein crystallization, purification, or chromatography: Suitable for crystallization trials, chromatography, and protein purification workflows.
-
Protein quantification assays: Compatible with Bicinchoninic Acid (BCA) Assay for accurate protein concentration measurement.
Safety Disclaimer
This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
