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$276.13
Sku: NKB-501
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by June 13, 2026
Description
Can Get Signal™ immunostain is a reaction solution that contains an accelerator for antigen-antibody reactions, which improves sensitivity, specificity, and S/N of immunohistochemistry (IHC) and immunocytochemistry.
Features
- • Improves sensitivity, specificity, and S/N of IHC.
- • Can be applied to various detection systems (e.g., chromogenic, chemiluminescence, or fluorescence).
- • Can be used with ABC or polymer complex methods.
- • Solutions A and B exhibit various properties for improving results.
- • Can be used directly without dilution (Ready-to-use).
Application
・Immunohistochemistry (IHC)
・Immunocytochemistry
Specification
| Category | Item | Details |
|---|---|---|
| Storage condition | - | Store at 4ºC |
Components
This kit includes the following components. All reagents should be stored at 4ºC and protected from light.
| Reagent Name | Code No. | ||
|---|---|---|---|
| NKB-401 | NKB-501 | NKB-601 | |
| Solution A | 5 mL | 20 mL | - |
| Solution B | 5 mL | - | 20 mL |
Typical reaction flow
Flow chart of immunostaining with Can Get Signal™ immunostainApplication Data
Example 1.Detection of PCNA using paraffin-embedded sections
The localization of PCNA (proliferating cell nuclear antigen) expression in human skin was detected using paraffin-embedded sections of the human skin tissue model TESTSKINTM (Toyobo). Detection was performed by the ABC method with anti-PCNA mouse monoclonal antibody as the primary antibody and biotinylated mouse IgG as the secondary antibody. Each antibody was diluted with Solution A of Can Get Signal™ immunostain prior to use. As a control experiment, PBS(-) containing 1.5% normal horse serum (conventional method) was used instead of Solution A of Can Get Signal™ immunostain. As a result, Can Get Signal™ immunostain produced higher signals and lower background than the conventional method.
Fig. 1. IHC detection of PCNA using paraffin-embedded tissue sections
Example 2. Detection of paxillin with the fluorescent antibody method
Fig. 2. Immunocytochemistry detection of paxillin with the fluorescent antibody method
*The data was kindly provided by Dr. Harada, Tokyo Institute of Technology.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
