C57BL/6 Mouse Primary Liver Epithelial Cells

Suggested Medium

M6621 Complete Epithelial Cell Medium w/ Kit – 500 ml

Description

C57BL/6 Mouse Primary Liver Epithelial Cells  are isolated from the liver tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. C57BL/6 Mouse Primary Liver Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Features

• High Purity, Pathogen‑Free Source: Isolated from SPF C57BL/6 mice for consistent experimental reproducibility.

• Cryopreserved & Ready‑to‑Use Cells: Delivered frozen for immediate integration into your research workflow.

• Quality Controlled: Negative for contamination (mycoplasma, bacteria, yeast, fungi) with validated epithelial markers.

• Expandable: Can be cultured and expanded for multiple passages under recommended conditions.

• Reliable Handling & Storage: Shipped on dry ice, store in liquid nitrogen for long‑term use.

Application

Specifically optimized for in vitro cell biology applications, these C57BL/6 Mouse Primary Liver Epithelial Cells are suitable for:

• Cell‑Cell Interaction & Adhesion Assays – Ideal for investigating epithelial behavior, tissue modeling, and cell adhesion properties.

• Western Blot (WB), PCR & Gene Expression Profiling – Excellent for downstream molecular biology analyses.

• Immunofluorescence & Flow Cytometry – Perfect for phenotypic characterization and marker analysis.

• Immunoprecipitation (IP) & Functional Assays – Supports protein interaction studies and signaling research.

• Advanced Culture Models – Use in co‑culture systems, primary tissue models, and drug screening platforms.

These versatile cells are essential for studies in hepatocyte biology, epithelial signal transduction, toxicology screening, and liver disease modeling. Suitable for academic, pharmaceutical, and biotech research use.

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Disclaimer

For Research Use Only

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.