C57BL/6 Mouse Lung Mesenchymal Stem Cells

Description

Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶ cells per ml and is delivered frozen. Mouse Lung Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended. Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by Cell Biologics.

Suggested Medium

  Mouse Mesenchymal Stem Cell Medium /w Kit - 500 ml

Catalog No. M5567

Custom order (2-4 weeks)

Laboratory Applications

Mouse Lung Mesenchymal Stem Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent flow cytometry. 

Storage

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Documents

Cell-Culture-Protocol-CB

Disclaimer

Although Mouse Lung Mesenchymal Stem Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution, since no test procedure can completely guarantee the absence of infectious agents.