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Specification
| Package | 500 U |
| Full name | BspQI |
| Applications | It is commonly used in experiments such as Golden Gate assembly, rapid cloning, and preparation of a large number of linearized plasmids. |
| Summary | BspQI is the isoschizome of the SpeedyCut series restriction enzyme SapI, but because the reaction buffer is consistent with the BsmBI enzyme reaction system, the BspQI endonuclease can exert maximum activity, so it can be shared with the B600199 reaction buffer, but not with the general SpeedyOne Buffer, and the recombinant albumin component is added to the reaction buffer, which greatly improves the stability of the enzyme |
| Features | BspQI is a type of Type IIs endonuclease that recognizes non-palindromic sequences and cuts them outside of the recognition sequence |
| Composition | 50ul BspQI, 10X BspQI buffer (dedicated) |
| Recognition Site | 5'…-GCTCTTC(N)1↓…-3' 3'…-CGAGAAG(N)4↑…-5' |
| Reaction Buffer | 10X BspQI Buffer (Dedicated) |
| Recommended Reaction Temperature | 50°C |
| Unit Definition | One unit of activity refers to the amount of enzyme required to completely digest 1 μg of λDNA in a 50 μl reaction system at 50℃ for 1 hour. |
| Methylation Effects | Dam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect. |
| Thermal Inactivation | Heat at 80°C for 20 min to lose activity |
| Component | 50ul BspQI, 10X BspQI buffer (dedicated) |
Storage
-20°C
Documents
https://cdn.shopifycdn.net/s/files/1/0521/5312/2997/files/P310002_EN_P.pdf
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