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Description
Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Specifications
| Grade | High Purity |
| Formula | C14H16BrNO6 |
| MW | 374.2 |
| CAS | 97753-82-7 |
| Appearance | White to Off-white Powder |
| Color of Solution (4% in DMF) | Colorless |
| Turbidity (0.4% in DMF) | ≤2.0 NTU |
| Water Content (K. Fischer) | ≤0.5% |
| Purity(HPLC) | ≥99.0 area-% |
| TLC (50mg in 1ml DMF and 9ml propanol; butanol/water 86:14;UV 254nm, sulfuric acid) |
Corresponds to reference material |
| Specific Optical Rotation [alpha]D (c=1 in DMF/water 1:1; 20o C) |
-35.0 to -32.0 |
| FT-IR Spectrum | Corresponds to reference spectrum |
| Storage | -15°C- -20°C under Nitrogen in dark |
Application
Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.
Documents
Disclaimer: For laboratory research use only.
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