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$365.00
Sku: BFMSKINO100
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by November 20, 2026
Background
Three-dimensional organoid culture has moved from a specialist technique to a standard model system. Primary-tissue organoids retain the cell-type composition, polarity and architecture of the tissue they came from, which two-dimensional culture loses within days — and that gap is why regulators and funders are now pushing non-animal models for toxicology and disease work.
Skin organoid culture has to support an epithelial compartment and its appendage lineages together. A keratinocyte medium expands cells but does not produce a stratified, appendage-competent organoid, and skin organoid media are not widely sold.
This kit supplies that formulation as two components. Primary tissue goes in, organoids come out, and the composition is the same from lot to lot. Building an equivalent medium in-house from individual niche factors is expensive and hard to keep consistent between batches.
Description
Primary tissue to organoid. Biofargo Mouse Skin Organoid Medium Kit is a serum-free, defined culture system for the establishment, expansion and long-term maintenance of mouse skin organoids in three-dimensional matrix culture. It is supplied as a basal medium plus a concentrated culture additive, mixed before use.
The kit is designed for the full workflow rather than one step of it: organoid generation from freshly dissociated primary mouse skin tissue, stable maintenance through passaging, and expansion of established lines. The defined, serum-free composition removes serum lot variation as a source of experimental drift, which matters when organoid cultures are carried for weeks.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Specifications
| Parameter | Specification |
| Product Name | Biofargo Mouse Skin Organoid Medium Kit |
| Catalog Number | BFMSKINO100 |
| Product Type | Serum-free, defined mouse skin organoid medium kit |
| Starting Material | Primary mouse skin tissue |
| Pack Size | 1 kit (100 mL complete medium) |
| Components | Basal medium 96 mL + culture additive 4 mL |
| Culture Format | 3D culture with extracellular matrix embedding |
| Culture Condition | 37 °C, 5% CO2 |
| Workflow Supported | Establishment, long-term maintenance and expansion |
| Serum | Serum-free |
| Storage | Basal medium 2–8 °C; culture additive −20 °C |
| Shipping | Shipped with cold packs |
| Intended Use | For research use only |
Features
Primary tissue to organoid in one kit — establishment, maintenance and expansion are covered by the same medium, so there is no switch between an establishment and a growth formulation partway through a culture.
Serum-free and defined — removes serum lot variation, which is the usual source of drift in organoid cultures carried for weeks.
Two-component format — basal medium plus concentrated additive; mix once and the complete medium is ready, with no multi-factor cocktail to assemble at the bench.
Epidermal identity retained — organoids cultured in this medium retain tissue-specific marker expression rather than dedifferentiating, which is the practical advantage over two-dimensional culture.
Works with standard matrix embedding — compatible with routine basement-membrane matrix workflows and standard plate formats.
Species- and tissue-matched — formulated for mouse skin tissue specifically, not a general-purpose organoid medium applied to every tissue.
Performance Data
Organoid formation and expansion from primary tissue
Following skin tissue isolation, cell collection and matrix embedding, organoid structures were observed during culture progression in Biofargo Mouse Skin Organoid Medium Kit, and cultures reached a stage suitable for passaging and continued expansion.
Tissue-specific marker expression
Marker analysis of organoids cultured in this medium showed expression patterns associated with epidermal identity, confirming that tissue character is retained through culture rather than lost to dedifferentiation. Markers examined included KRT1, involucrin and loricrin.
Applications
Wound healing and regeneration: Skin organoids support study of epidermal repair, keratinocyte migration and re-epithelialisation in three dimensions.
Hair follicle and appendage biology: Appendage lineages are retained in organoid culture, which monolayer keratinocyte culture does not support.
Dermal toxicity and irritation: A three-dimensional skin model for compound and formulation toxicity, relevant where animal testing for cosmetics is restricted.
Skin disease modelling: Generate organoids from genetically modified or treated animals to study barrier defects and inflammatory skin disease.
Epidermal stem cell biology: Study basal stem cell self-renewal, commitment and stratification.
Organoid expansion and biobanking: Maintain and expand established lines for repeated downstream assays from a single tissue harvest.
Organoid models complement, rather than replace, in vivo work. Findings should be confirmed in the model system appropriate to the research question.
Kit Components
| Component | Volume | Storage |
| Mouse Skin Organoid Basal Medium | 96 mL | 2–8 °C |
| Mouse Skin Organoid Culture Additive | 4 mL | −20 °C |
Storage & Shipping
Shipped with cold packs.
Store the basal medium at 2–8 °C and the culture additive at −20 °C.
Mix the additive into the basal medium to prepare complete medium. Aliquot the additive to avoid repeated freeze-thaw cycles.
Prepare only the volume of complete medium needed for near-term use and keep it at 2–8 °C.
Protocol Overview
Collect mouse skin tissue and wash in PBS.
Dissociate the tissue and prepare skin tissue or tissue-derived cells.
Filter and collect cells after digestion.
Embed the cells in basement-membrane matrix for three-dimensional culture.
Prepare complete medium by mixing the culture additive into the basal medium, and overlay the matrix.
Culture at 37 °C, 5% CO2, replacing the complete medium on the documented schedule.
Passage when organoids have reached a size suitable for dissociation and re-embedding.
Frequently Asked Questions (FAQs)
Common questions about Biofargo Mouse Skin Organoid Medium Kit.
What is this kit used for?
It is a serum-free, defined medium kit for establishing, maintaining and expanding mouse skin organoids from primary mouse skin tissue in three-dimensional matrix culture.
Does it start from primary tissue or from stem cells?
Primary tissue. Organoids are established from freshly dissociated primary mouse skin tissue, not from pluripotent stem cells. Tissue dissociation, filtration and matrix embedding precede the first medium change.
Is the medium serum-free?
Yes. The formulation is serum-free and defined, which removes serum lot variation as a source of experimental drift across long cultures.
What is in the kit, and how is complete medium prepared?
The kit contains 96 mL of basal medium and 4 mL of concentrated culture additive. Mix the additive into the basal medium to give 100 mL of complete medium. Prepare only what is needed in the near term and keep it at 2–8 °C.
Can the medium support expansion and passaging, not just establishment?
Yes. The same medium covers establishment, long-term maintenance and expansion, so there is no change of formulation partway through a culture.
What matrix and culture conditions are required?
Cells are embedded in basement-membrane matrix and cultured at 37 °C with 5% CO2. The matrix is not included; a growth-factor-reduced, phenol red-free basement membrane matrix is available separately.
Do the organoids retain tissue identity?
Marker analysis of organoids cultured in this medium shows expression patterns associated with epidermal identity. This is the practical advantage over two-dimensional culture, where tissue-specific function is lost within days.
Can organoids be cryopreserved and recovered?
Yes. Established lines can be banked with a protein-free organoid cryopreservation solution and recovered into this medium, so one tissue harvest supports repeated experiments over time.
How is it stored and shipped?
Shipped with cold packs. Store the basal medium at 2–8 °C and the culture additive at −20 °C.
Can it be used for clinical or diagnostic purposes?
No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.
Related Products
Basement Membrane Matrix, Growth Factor Reduced, Phenol Red-Free — matrix for 3D embedding; phenol red-free for fluorescence readouts.
Organoid Tissue Digestion Solution — for dissociating primary tissue before embedding.
OrgCryo Protein-Free Organoid Cryopreservation Solution — for banking established organoid lines.
All organoid culture media — liver, intestinal, lung, gastric, colon, kidney and skin kits in the same format.
Documents
Instruction manual, SDS and lot-specific COA are available on request. Please contact us with the catalog number and lot number.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
