Binding/Wash Buffer For Ni-NTA/Ni-IDA,10 mM Imidazole pH 7.9~8.1

Description

Biofargo Binding & Wash Buffer for Ni-NTA / Ni-IDA is a ready-to-use, precisely formulated buffer designed for His-tag protein purification by immobilized metal affinity chromatography (IMAC).
Containing 10 mM imidazole at pH 7.9–8.1, this buffer effectively reduces non-specific protein binding while maintaining strong and selective interaction between His-tagged proteins and Ni-NTA or Ni-IDA resins.

Optimized for reliability and reproducibility, this buffer is fully compatible with gravity flow columns, spin columns, syringe formats, and FPLC systems, making it ideal for routine laboratory purification, method development, and scale-up workflows.

Specifications

Features

• Optimized 10 mM Imidazole Concentration

  Minimizes non-specific binding without disrupting His-tag–metal affinity interactions.

• Controlled pH 7.9–8.1

  Ensures optimal binding efficiency and protein stability during IMAC purification.

• Compatible with Ni-NTA & Ni-IDA Resins

  Suitable for all standard nickel-based affinity chromatography matrices.

• High Ionic Strength Formulation

  Reduces background proteins and improves purification selectivity.

• Ready-to-Use Format

  Eliminates buffer preparation time and reduces variability between experiments.

• Reproducible & Scalable

  Suitable for research, process development, and preparative purification workflows.

Applications

This buffer is designed for use in His-tag affinity purification workflows, including:

• Binding and wash steps in Ni-NTA / Ni-IDA IMAC purification

• Removal of weakly or non-specifically bound host proteins

• Recombinant protein purification from bacterial, yeast, or mammalian lysates

• Gravity flow columns, spin columns, and automated FPLC systems

• Protein expression screening and downstream process optimization

Typical Workflow Compatibility

• Column equilibration

• Sample loading

• Binding / Wash with 10 mM Imidazole Buffer

• Elution with higher imidazole concentration buffer

• This product is specifically optimized for Step 3, ensuring cleaner elution profiles and improved downstream purity.

Usage Instructions

Compatible with equilibration buffers and wash buffers for Ni-NTA/IDA resin purification. The imidazole concentration in the wash buffer depends on the protein sample. For most contaminating proteins, a concentration of 10-20 mM imidazole is typically recommended. For Ni-TED resin, use with product C600500.

Components

Components include: 10 mM Imidazole, NaCl, NaH2PO4·2H2O, pH 7.9~8.1

Why Choose Biofargo?

Biofargo provides high-quality, application-validated buffers designed to meet the needs of modern protein purification workflows.

Each formulation is developed with a focus on performance consistency, practical usability, and cost efficiency, making it a dependable alternative to premium brand buffers—without compromising results.