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Sku: BF-SNU466
Description
Laboratories selecting a human glioblastoma cell line need clearly documented information on tissue origin, cellular morphology, growth pattern, culture medium, passaging, and cell identity before beginning routine culture. BF-SNU466 is a human brain-derived glioblastoma cell line established from a 61-year-old Asian male. The cells have spindle morphology and grow as a monolayer.
The BF-SNU466 cell line has a reported population doubling time of 54 hours and a recommended split ratio of 1:4. Its specified culture system uses RPMI 1640 containing L-glutamine, HEPES, and sodium bicarbonate, supplemented with fetal bovine serum. The documented culture conditions, subculturing procedure, freezing medium, and STR profile below provide laboratories with the reference information needed to plan the routine handling of BF-SNU466 cells.
Specification
| Product Name | BF-SNU466 Human Glioblastoma Cell Line |
|---|---|
| Catalog Number | BF-SNU466 |
| Species | Human |
| Donor | Asian male, 61 years old |
| Tissue of Origin | Brain |
| Histopathology | Glioblastoma |
| Cellular Morphology | Spindle |
| Growth Pattern | Monolayer |
| Population Doubling Time | 54 hours |
| Split Ratio | 1:4 |
| Original Culture Medium | RPMI 1640 containing L-glutamine (300 mg/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10% |
| Culture Medium | RPMI 1640 containing L-glutamine (300 mg/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; fetal bovine serum, 10% |
| Freeze Medium | RPMI 1640, 52.5%; FBS, 40%; DMSO, 7.5% |
| Media Change | Every 2 to 3 days, depending on cell density |
| Depositor | Jae-Gahb Park, M.D. |
BF-SNU466 STR Profile
| STR Locus | Alleles |
|---|---|
| D3S1358 | 15, 18 |
| vWA | 17, 18 |
| FGA | 22, 24 |
| Amelogenin | X, Y |
| TH01 | 6, 9 |
| TPOX | 9, 11 |
| CSF1P0 | 12 |
| D5S818 | 7, 12 |
| D13S317 | 8, 12 |
| D7S820 | 10, 12 |
Features
- Human glioblastoma cell line
- Derived from brain tissue
- Donor was a 61-year-old Asian male
- Spindle cellular morphology
- Monolayer growth pattern
- Reported population doubling time of 54 hours
- Recommended split ratio of 1:4
- RPMI 1640-based culture medium
- Documented 10-locus STR profile
Application
BF-SNU466 cells are intended for research involving a human brain-derived glioblastoma cell line with spindle morphology and a monolayer growth pattern.
Subculturing Procedure
- Remove the culture medium.
- Add fresh 0.25% trypsin and 0.02% EDTA solution.
- Keep the culture flask at 37°C for 3 to 5 minutes.
- Add culture medium and collect the cells.
- Transfer the cell suspension into a 15 mL tube and centrifuge.
- Aspirate the medium.
- Resuspend the cell pellet with culture medium and dispense the cells into a culture flask.
Media Change
Remove two-thirds of the medium and add fresh medium every 2 to 3 days, depending on cell density.
FAQ
What is the BF-SNU466 cell line?
BF-SNU466 is a human glioblastoma cell line derived from brain tissue. The cells have spindle morphology and grow as a monolayer.
What is the catalog number for this human glioblastoma cell line?
The catalog number is BF-SNU466.
What is the origin of BF-SNU466 cells?
BF-SNU466 cells were derived from the brain of a 61-year-old Asian male. The reported histopathology is glioblastoma.
What morphology do BF-SNU466 cells have?
BF-SNU466 cells have spindle cellular morphology and a monolayer growth pattern.
What medium is used to culture BF-SNU466 cells?
The specified culture medium contains 90% RPMI 1640 with L-glutamine at 300 mg/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% fetal bovine serum.
What original medium is specified for BF-SNU466 cells?
The specified original medium contains 90% RPMI 1640 with L-glutamine at 300 mg/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.
What is the population doubling time of BF-SNU466 cells?
The reported population doubling time of the BF-SNU466 cell line is 54 hours.
What split ratio is recommended for BF-SNU466 cells?
The recommended split ratio for BF-SNU466 cells is 1:4.
How often should the BF-SNU466 culture medium be changed?
Two-thirds of the medium should be removed and replaced with fresh medium every 2 to 3 days, depending on cell density.
How are BF-SNU466 cells subcultured?
Remove the medium and add fresh 0.25% trypsin with 0.02% EDTA. Keep the flask at 37°C for 3 to 5 minutes, add culture medium, and collect the cells. Transfer the suspension to a 15 mL tube, centrifuge, aspirate the medium, resuspend the pellet, and dispense the cells into a culture flask.
What freezing medium is specified for BF-SNU466 cells?
The specified freeze medium contains 52.5% RPMI 1640, 40% FBS, and 7.5% DMSO.
Is an STR profile available for BF-SNU466 cells?
Yes. The documented loci are D3S1358, vWA, FGA, Amelogenin, TH01, TPOX, CSF1P0, D5S818, D13S317, and D7S820. The corresponding allele values are provided in the specification table.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
