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Sku: BF-SNU333
Description
Laboratories working with human renal cell carcinoma cells need clearly documented information on tissue origin, morphology, growth pattern, culture medium, passaging, and cell identity before beginning routine culture. The BF-SNU333 cell line is derived from the kidney of a 34-year-old Asian male with renal cell carcinoma. These human kidney cancer cells display epithelial morphology and grow as a monolayer.
This BF-SNU333 human renal cell carcinoma cell line, catalog number BF-SNU333, has a reported population doubling time of 76 hours and a recommended split ratio of 1:3. The specified original culture medium consists of 90% RPMI 1640 containing L-glutamine, HEPES, and sodium bicarbonate, supplemented with 10% heat-inactivated fetal bovine serum. The documented culture information and STR profile below allow researchers to review the characteristics of BF-SNU333 cells before use.
Specification
| Product Name | BF-SNU333 Cell Line |
|---|---|
| Catalog Number | BF-SNU333 |
| Species | Human |
| Donor | Asian male, 34 years old |
| Tissue of Origin | Kidney |
| Histopathology | Renal cell carcinoma |
| Cellular Morphology | Epithelial |
| Growth Pattern | Monolayer |
| Population Doubling Time | 76 hours |
| Split Ratio | 1:3 |
| Original Culture Medium | RPMI 1640 containing L-glutamine (300 mg/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10% |
| Freeze Medium | RPMI 1640, 52.5%; FBS, 40%; DMSO, 7.5% |
| Media Change | Every 2 to 3 days, depending on cell density |
| Depositor | Jae-Gahb Park, M.D. |
BF-SNU333 STR Profile
| STR Locus | Alleles |
|---|---|
| D3S1358 | 17 |
| vWA | 17 |
| FGA | 22, 23 |
| Amelogenin | X, Y |
| TH01 | 7, 9 |
| TPOX | 8 |
| CSF1P0 | 10, 11 |
| D5S818 | 11, 13 |
| D13S317 | 11 |
| D7S820 | 9, 13 |
Features
- Human renal cell carcinoma cell line
- Derived from kidney tissue
- Epithelial cellular morphology
- Monolayer growth pattern
- Reported population doubling time of 76 hours
- Recommended split ratio of 1:3
- RPMI 1640-based original culture medium
- Documented 10-locus STR profile
Application
BF-SNU333 cells are intended for research involving a human kidney-derived renal cell carcinoma cell line with epithelial morphology and a monolayer growth pattern.
Subculturing Procedure
- Remove the culture medium.
- Add fresh 0.25% trypsin and 0.02% EDTA solution.
- Keep the culture flask at 37°C for 3 to 5 minutes.
- Add culture medium and collect the cells.
- Transfer the medium into a 15 mL tube and centrifuge.
- Aspirate the medium.
- Resuspend the cell pellet with culture medium and dispense it into the culture flask.
Media Change
Remove two-thirds of the medium and add fresh medium every 2 to 3 days, depending on cell density.
FAQ
What is the BF-SNU333 cell line?
BF-SNU333 is a human renal cell carcinoma cell line derived from kidney tissue. The cells have epithelial morphology and grow as a monolayer.
What is the catalog number for the BF-SNU333 cell line?
The catalog number for this BF-SNU333 cell line is BF-SNU333.
What is the origin of BF-SNU333 cells?
BF-SNU333 cells were derived from the kidney of a 34-year-old Asian male. The reported histopathology is renal cell carcinoma.
What morphology and growth pattern do BF-SNU333 cells have?
BF-SNU333 cells have epithelial cellular morphology and a monolayer growth pattern.
What medium is specified for culturing BF-SNU333 cells?
The specified original medium is 90% RPMI 1640 containing L-glutamine at 300 mg/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.
What is the population doubling time of BF-SNU333 cells?
The reported population doubling time of the BF-SNU333 cell line is 76 hours.
What split ratio is recommended for BF-SNU333 cells?
The recommended split ratio for BF-SNU333 cells is 1:3.
How often should the BF-SNU333 culture medium be changed?
Two-thirds of the medium should be removed and replaced with fresh medium every 2 to 3 days, depending on cell density.
How are BF-SNU333 cells subcultured?
Remove the medium and add fresh 0.25% trypsin with 0.02% EDTA. Keep the flask at 37°C for 3 to 5 minutes, add culture medium, and collect the cells. Transfer the suspension to a 15 mL tube, centrifuge, aspirate the medium, resuspend the pellet, and dispense the cells into a culture flask.
What freezing medium is specified for BF-SNU333 cells?
The specified freeze medium contains 52.5% RPMI 1640, 40% FBS, and 7.5% DMSO.
Is an STR profile available for BF-SNU333 cells?
Yes. The documented loci are D3S1358, vWA, FGA, Amelogenin, TH01, TPOX, CSF1P0, D5S818, D13S317, and D7S820. The corresponding allele values are provided in the specification table.
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