0 item(s)
View cart
You have no items in your shopping cart.
Request Your Custom Quote Today
Tell us your required quantity and application needs to receive a tailored quotation for this product.
Sku: BF-HCC2108
Description
Laboratories selecting a human lung adenocarcinoma cell line need clearly documented information on tissue origin, cellular morphology, growth pattern, culture medium, freezing medium, and cell identity before beginning routine culture. BF-HCC2108 is a human lung-derived adenocarcinoma cell line established from a 59-year-old male. The cells have epithelial morphology and grow as a monolayer.
The BF-HCC2108 cell line uses an RPMI 1640-based culture medium supplemented with heat-inactivated fetal bovine serum. The documented culture information includes the medium formulation, freezing medium, media-change interval, available subculture information, and a 10-locus STR profile. These specifications allow laboratories to review the reported characteristics of BF-HCC2108 cells before use.
Specification
| Product Name | BF-HCC2108 Human Lung Adenocarcinoma Cell Line |
|---|---|
| Catalog Number | BF-HCC2108 |
| Species | Human |
| Donor | Male, 59 years old |
| Tissue of Origin | Lung |
| Histopathology | Adenocarcinoma |
| Cellular Morphology | Epithelial |
| Growth Pattern | Monolayer |
| Original Culture Medium | RPMI 1640 containing L-glutamine (300 mg/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10% |
| Culture Medium | RPMI 1640 containing L-glutamine (300 mg/L), 25 mM HEPES, and 25 mM NaHCO3, 90%; heat-inactivated fetal bovine serum, 10% |
| Freeze Medium | RPMI 1640, 52.5%; FBS, 40%; DMSO, 7.5% |
| Media Change | Every 2 to 3 days, depending on cell density |
| Available Subculture Information | Remove the medium, add fresh 0.25% trypsin and 0.02% EDTA solution, and keep the culture flask at 37°C. Additional subculture steps are not listed in the provided product information. |
| Depositor | Gazdar AF |
BF-HCC2108 STR Profile
| STR Locus | Alleles |
|---|---|
| D3S1358 | 15 |
| vWA | 16 |
| FGA | 22 |
| Amelogenin | X |
| TH01 | 7, 9 |
| TPOX | 10, 11 |
| CSF1P0 | 11 |
| D5S818 | 11, 13 |
| D13S317 | 11 |
| D7S820 | 9, 11 |
Features
- Human lung adenocarcinoma cell line
- Derived from lung tissue
- Donor was a 59-year-old male
- Epithelial cellular morphology
- Monolayer growth pattern
- RPMI 1640-based culture medium
- Specified freezing medium containing RPMI 1640, FBS, and DMSO
- Media change every 2 to 3 days, depending on cell density
- Documented 10-locus STR profile
Application
BF-HCC2108 cells are intended for research involving a human lung-derived adenocarcinoma cell line with epithelial morphology and a monolayer growth pattern.
Culture Information
The specified culture medium contains 90% RPMI 1640 with L-glutamine at 300 mg/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.
Media Change
Remove two-thirds of the medium and add fresh medium every 2 to 3 days, depending on cell density.
Available Subculture Information
Remove the culture medium, add fresh 0.25% trypsin and 0.02% EDTA solution, and keep the culture flask at 37°C. Additional subculture steps are not listed in the provided product information.
FAQ
What is the BF-HCC2108 cell line?
BF-HCC2108 is a human lung adenocarcinoma cell line derived from lung tissue. The cells have epithelial morphology and grow as a monolayer.
What is the catalog number for this human lung adenocarcinoma cell line?
The catalog number is BF-HCC2108.
What is the origin of BF-HCC2108 cells?
BF-HCC2108 cells were derived from the lung of a 59-year-old male. The reported histopathology is adenocarcinoma.
What morphology do BF-HCC2108 cells have?
BF-HCC2108 cells have epithelial cellular morphology and a monolayer growth pattern.
What medium is used to culture BF-HCC2108 cells?
The specified medium contains 90% RPMI 1640 with L-glutamine at 300 mg/L, 25 mM HEPES, and 25 mM NaHCO3, supplemented with 10% heat-inactivated fetal bovine serum.
What freezing medium is specified for BF-HCC2108 cells?
The specified freezing medium contains 52.5% RPMI 1640, 40% FBS, and 7.5% DMSO.
How often should the BF-HCC2108 culture medium be changed?
Two-thirds of the medium should be removed and replaced with fresh medium every 2 to 3 days, depending on cell density.
What subculture information is available for BF-HCC2108 cells?
The available information states that the medium should be removed, fresh 0.25% trypsin and 0.02% EDTA solution should be added, and the culture flask should be kept at 37°C. Additional subculture steps are not listed in the provided product information.
Is a doubling time specified for BF-HCC2108 cells?
No population doubling time is listed in the provided product information.
Is a split ratio specified for BF-HCC2108 cells?
No recommended split ratio is listed in the provided product information.
Is an STR profile available for BF-HCC2108 cells?
Yes. The documented loci are D3S1358, vWA, FGA, Amelogenin, TH01, TPOX, CSF1P0, D5S818, D13S317, and D7S820. The corresponding allele values are provided in the specification table.
What Amelogenin result is reported for BF-HCC2108 cells?
The reported Amelogenin result is X.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
