BCA Protein Quantification Kit

Description

BCA Protein Quantification Kit is a sensitive and reliable protein assay kit designed for accurate total protein concentration determination in biological samples. During protein research workflows, inconsistent protein input amounts can affect downstream experiments such as Western blot, SDS-PAGE, immunoprecipitation, and biochemical analysis. Reliable protein quantification is therefore essential for sample normalization and experimental reproducibility.

This BCA Protein Assay Kit utilizes the bicinchoninic acid (BCA) colorimetric assay method to measure protein concentration through copper ion reduction and BCA-Cu+ complex formation. The generated purple color signal is proportional to protein concentration and can be quantified by measuring absorbance at 562 nm using a microplate reader or spectrophotometer.

With a broad detection range, high sensitivity, and excellent compatibility with common detergents, this protein quantification kit provides a convenient solution for cell lysates, tissue extracts, and purified protein samples. The kit is suitable for routine protein concentration measurement before Western blot, SDS-PAGE, and other protein analysis workflows.

Specifications

Features

• Accurate total protein quantification: Provides reliable measurement of protein concentration using the widely used BCA assay method.

• High sensitivity detection: Detects protein concentrations down to 25 μg/mL with a minimum detection amount of 0.5 μg protein.

• Broad sample compatibility: Suitable for cell lysates, tissue extracts, purified proteins, and common biological samples.

• Detergent compatible protein assay: Compatible with commonly used detergents including SDS, Triton X-100, and Tween series at appropriate concentrations.

• Convenient standard curve preparation: Includes ready-to-use protein standard solution for simplified BCA protein assay workflows.

• Microplate-based protein detection: Supports high-throughput protein quantification using 96-well plates and microplate readers.

Applications

BCA Protein Quantification Kit is widely used for protein concentration measurement in molecular biology, cell biology, and biochemical research applications.

• Western Blot Sample Normalization: Determine protein concentration before SDS-PAGE loading to ensure consistent protein input between samples.

• Cell Lysate Protein Quantification: Measure total protein concentration from cultured cell extracts prepared with common lysis buffers.

• Tissue Protein Extraction Analysis: Quantify protein content from animal tissue lysates and complex biological samples.

• SDS-PAGE Sample Preparation: Normalize protein amounts before electrophoresis for accurate protein comparison.

• Immunoprecipitation Sample Preparation: Determine protein concentration before antibody-based enrichment workflows.

• Protein Purification Analysis: Monitor protein recovery and concentration during purification procedures.

• Recombinant Protein Quantification: Measure purified protein samples for downstream biochemical experiments.

• Biochemical Research: Suitable for general protein concentration determination in laboratory research workflows.

Protocol Overview

The BCA Protein Quantification Kit provides a simple colorimetric workflow for protein concentration measurement.

• Prepare protein standards using the supplied protein standard solution.

• Add protein samples and standards into a 96-well plate.

• Prepare BCA working reagent by mixing BCA Reagent A and BCA Reagent B at a 50:1 ratio.

• Add BCA working reagent and incubate samples at 37°C for approximately 30 minutes.

• Measure absorbance at 562 nm and calculate protein concentration using the standard curve.

For accurate protein concentration determination, it is recommended to generate a standard curve for each experiment because BCA color development can be affected by incubation time and temperature.

Ordering Information

FAQ