BAV-3 Analysis Kit SHENTEK®

For Research Use Only (RUO)

$1,010.00

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Sku: 1506734
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Availability:
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In stock & estimated to ship in 3-7 days by May 12, 2026
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Analysis Method
qPCR
Assay Time
Refer to manual
Storage Temperature
-20 °C (stable for 24 months)

Product Description

The SHENTEK® BAV-3 Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.

Technical Specifications

Parameter Details
Limit of detection (LOD) 50 copies per reaction
Assay type Qualitative probe-based real-time PCR
Reaction format / volume Per reaction: 20 µL qPCR MIX + 10 µL sample or control = 30 µL total
qPCR mix composition (per reaction) BAV-3 Primer & Probe MIX 2.8 µL; qPCR Reaction Buffer 15.9 µL; IPC MIX 1.3 µL; total 20 µL
qPCR cycling conditions Activation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (45 cycles); Annealing/extension 60 °C 01:00 (read fluorescence during this step)
Instrument compatibility SHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and similar real-time PCR instruments
Sample input (recommended) Cell culture or bovine serum: 100–200 µL (≤10^7 cells). Follow Virus DNA & RNA Extraction Kit protocol for extraction.
Control acceptance criteria (general) NTC: FAM Ct ≥ 40 or no amplification; VIC Ct < 35 indicates IPC amplification. PCS: Ct < 35 (FAM and VIC). See manual tables for full decision matrix.
Specificity No cross-amplification observed with bovine, porcine genomes; common production cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris).
Kit capacity / reactions Reagents for 50 reactions
Shelf life / stability Kit components can be stored under appropriate conditions for up to 24 months (see storage information).

Features

  • High sensitivity: Assay sensitivity down to 50 copies per reaction.
  • High specificity: No cross-amplification with tested bovine/porcine genomes, common cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris).
  • Integrated internal control: Includes IPC MIX to monitor extraction and PCR inhibition (VIC channel).
  • Optimized for complex matrices: Validated for detection of BAV-3 DNA from cell culture and bovine serum when used with SHENTEK® Virus DNA & RNA Extraction Kit.
  • Platform flexibility: Compatible with multiple real-time PCR instruments (SHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96, etc.).

Applications

  • Qualitative molecular analysis of BAV-3 target sequences in industrial cell banks
  • Analytical quality control of biological seed lots
  • Molecular evaluation of in vitro cell culture supplements for BAV-3 nucleic acids
  • In-process monitoring and lot-release analysis for biological manufacturing
  • In vitro research projects assessing BAV-3 target sequences in complex culture matrices

Kit Contents

BAV-3 Primer & Probe MIX (NNC094)-20 °C, protect from light (150 µL × 1 tube)
qPCR Reaction Buffer (NNB001)-20 °C (850 µL × 1 tube)
IPC MIX (NNC066)-20 °C (150 µL × 1 tube)
DNA Dilution Buffer (DDB) (NND001)-20 °C (1.5 mL × 1 tube)
* Total: 50 reactions | Method: qPCR

Attention

• For Research Use Only (RUO). Not for diagnostic or therapeutic use.

• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear PPE (protective eyewear, mask, clothing, gloves).

• Handle positive control and extraction steps in a biosafety cabinet; follow BSL-2 laboratory safety guidelines.

• Use nuclease-free consumables; avoid cross-contamination between samples, controls and reagents.

• Thaw kit components completely at 2–8 °C or on ice before use; vortex and briefly centrifuge.

• Protect primer & probe mix from light.

• If VIC (IPC) indicates inhibition, repeat sample preparation or remove inhibitors and repeat assay.

• Follow instrument-specific setup and thresholding as described in the manual; validate control acceptance criteria during method validation.

Quality Management & Certifications

Quality System

Download QMS

QMS (ISO, GMP)

Download Certificate

Quality Advantages

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Quality Control Process

Download Process

📂 Technical Resources & Downloads

PDF Product Manual ↓ PDF MSDS / SDS ↓

Frequently Asked Questions (FAQ)

Q1: What is the assay sensitivity (LOD)?
The assay sensitivity is 50 copies per reaction (LOD = 50 copies/reaction).
Q2: How many reactions are provided in one kit?
The kit provides reagents for 50 reactions.
Q3: What sample volume is recommended for cell culture or serum?
Collect 100–200 µL of cell culture (≤10^7 cells) or bovine serum per sample and follow the Virus DNA & RNA Extraction Kit protocol for nucleic acid extraction.
Q4: How should the kit components be stored?
Store kit components at −20 °C (stable for up to 24 months); protect primer/probe mix from light. Thaw at 2–8 °C or on ice before use.

Research Use Only

✔

Research Use Only (RUO) - Products are intended for research use only – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

✔

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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