For Research Use Only (RUO)

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⚡ Detection Method
ELISA
🕒 Assay Time
Refer to manual
🎯 Storage Temperature
-65 °C (stable for 12 months)

Product Description

SHENTEK Anti-P.pastoris HCP Antibody is an affinity-purified polyclonal sheep IgG raised against Pichia pastoris (Komagataella phaffii) host cell proteins (HCPs) produced by high-pressure homogenization. Supplied as an unconjugated liquid antibody in PBS without preservatives. Intended for use in HCP coverage evaluations and antibody-based methods including Western blot and ELISA. Supplied at 1 mg, validated for research applications. Short-term storage at 2-8°C; for long-term storage, keep below -65°C. Validity period 12 months.

Technical Specifications

Parameter Details
Size 1 mg
Species Reactivity Pichia pastoris (Komagataella phaffii)
Host/Isotype Sheep / IgG
Class Polyclonal
Type Antibody (unconjugated)
Conjugate Unconjugated
Immunogen P.pastoris HCPs produced by high-pressure homogenization
Form Liquid (in PBS)
Purification Affinity purified
Buffer PBS
Contains No preservatives
Applications HCP coverage evaluations, Western Blot, ELISA
Storage Maintain at 2-8°C for short-term use; for long-term storage store below -65°C
Validity period 12 months

Features

  • Broad HCP coverage: Polyclonal antibody raised against total P.pastoris HCPs to maximize detection of diverse host-cell proteins.
  • Affinity purified: Affinity purification improves specificity and reduces background in immunoassays.
  • Validated applications: Suitable for HCP coverage evaluations, Western blot and ELISA workflows.
  • High-quality immunogen preparation: Immunogen prepared by high-pressure homogenization to present native-like HCP profiles.
  • Preservative-free formulation: No preservatives in formulation for compatibility with downstream analytical assays; handle aseptically.

Applications

  • HCP coverage evaluation during antibody/assay development
  • Western blot analysis of P.pastoris host cell proteins
  • ELISA-based HCP detection and process monitoring
  • Process development and upstream/downstream HCP profiling

Kit Contents

Anti-P.pastoris HCP Antibody, 1 mg (liquid in PBS, unconjugated)Short-term 2-8°C; long-term below -65°C
* Total: Not applicable (antibody product) | Method: Binding assays (ELISA, Western Blot) — use as primary antibody

Attention

• For research use only. Not for diagnostic or therapeutic use.

• Contains no preservatives — handle under aseptic conditions.

• Avoid repeated freeze-thaw cycles; aliquot for long-term storage below -65°C.

• Maintain short-term storage at 2-8°C when in active use.

• Use appropriate laboratory protective equipment and follow institutional biosafety procedures.

Quality Management & Certifications

Quality System

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QMS (ISO, GMP)

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Quality Advantages

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Quality Control Process

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📂 Technical Resources & Downloads

Frequently Asked Questions (FAQ)

Q1: What are the recommended storage conditions and stability?
Short-term storage at 2-8°C. For long-term storage, store below -65°C. Product validity/stability period is 12 months.
Q2: Is this antibody conjugated?
No. This product is supplied unconjugated (sheep polyclonal IgG). It can be conjugated by the user to appropriate labels if required.
Q3: Which applications has this antibody been validated for?
Documented applications include HCP coverage evaluations, Western blot, and ELISA.
Q4: What is the immunogen and species reactivity?
The antibody was raised against P.pastoris host cell proteins (HCPs) produced by high-pressure homogenization and is reactive to Pichia pastoris (Komagataella phaffii).

Research Use Only

Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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