Animal Tissue Direct PCR Kit

Descprition

This product uses a unique lysis buffer system to rapidly release genomic DNA from animal tissue samples for PCR reactions, so it is especially suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C, and the released trace amount of DNA can be used as a template for PCR reaction without other processes such as protein removal and RNA removal.

2×PCR EasyTM Mix has a strong resistance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer, and stabilizer. It can be used in combination with lysis buffer to detect samples quickly and easily, and has the characteristics of high sensitivity, strong specificity and good stability.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions.

D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Features

• No need for time-consuming and expensive DNA purification.

• The sample requirement is small, as little as 5 mg of animal tissue can be used for experiments.

• No special treatment such as grinding and crushing is required, and the operation is simple.

• The optimized PCR system makes PCR more specific and more resistant to PCR reaction inhibitors.

Application

• Scope of application: a variety of animal tissues.

• DNA released from sample lysis: Use as PCR template only.

• The kit can be used for the following purposes: identification of transgenes, animal genotyping, etc.

Work Flow

Storage

• This kit Part I is stored at room temperature or 2-8℃.

• Reagent Buffer AL can be stored for 12 months under dry conditions; for longer storage, it can be stored at 2–8°C.

  Note: If stored at low temperature, the solution is prone to precipitation. Be sure to place the solution in the kit at room temperature for a period of time before use, and if necessary, preheat in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix well before use.

• Reagent Foregene Protease has a unique formula, in order to ensure its better activity and stability, please store it at 4℃.

• Reagent 6×DNA Loading Buffer, can be stored at 4°C or -20°C for a long time.

  This kit Part II is stored at -20°C.

• Reagent 2×PCR EasyTM Mix, if used frequently, it can also be stored at 4°C for a short time (limited to be used up within 10 days).

Documents

Animal Tissue Direct PCR Kit Instruction Manual-V1.0-EN