Alizarin Red S Indicator Grade

During osteogenic differentiation and mineralization studies, accurately evaluating calcium deposition is essential for confirming extracellular matrix mineralization and assessing cell differentiation outcomes. Conventional observation methods may not provide clear visualization of calcium-rich structures, affecting experimental interpretation and reproducibility.

Alizarin Red S is an anthraquinone-derived calcium staining reagent widely used for visualization of calcium deposits in biological samples. Through chelation with calcium ions, Alizarin Red S forms an Alizarin Red S-calcium complex that produces a distinct orange-red staining signal, enabling researchers to identify mineralized areas in tissue sections and cultured cells.

This research-grade Alizarin Red S staining reagent is commonly applied in osteogenic differentiation assays, bone biology research, mineralization assays, histological calcium staining, and cell-based calcium deposition detection workflows. The reagent provides a convenient solution for qualitative microscopic evaluation and colorimetric analysis of calcium mineralization.

Alizarin Red S staining solution is commonly prepared by dissolving 2 g of Alizarin Red S powder in 100 mL distilled water. The solution pH is adjusted to approximately 4.1–4.3 using ammonium hydroxide, as pH control is important for reliable calcium staining performance.

After staining, calcium deposits typically appear orange-red and demonstrate birefringent properties under microscopic observation.

For laboratory research use only.