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Description
ActCel T Cell Activation Reagent is synthesized by coupling a nanomatrix with anti-human CD3 antibody and anti-human CD28 antibody, and it can gently and efficiently activate T cells or PBMCs (Peripheral Blood Mononuclear Cells).
Specifications
| Reactive Species | Human |
| Endotoxin | <2EU/mL |
| Appearance | Brown liquid |
Instructions for Use
Experimental Procedures:
1.1 Conduct the experiment at a titer of 1:200. The specific addition amounts are shown in the following table:
| Culture Plate | Maximum Culture Volume | Total T Cell Number | Volume of T Cell Activation Reagent Added per Well |
| 96-well | 0.2 mL | 0.3×10⁶ | 1 μL |
| 48-well | 1 mL | 1×10⁶ | 5 μL |
| 24-well | 2 mL | 2×10⁶ | 10 μL |
| 12-well | 4 mL | 4×10⁶ | 20 μL |
| 6-well | 5 mL | 5×10⁶ | 25 μL |
1.2 Taking 1×10⁶ T cells as an example: Add 995 μL of X-VIVOTM 15 medium and 5 μL of T cell activation reagent (containing 200 IU/mL IL-2) to 1×10⁶ T cells. Then culture them at 37 °C under 5% CO₂ for 2 days.
1.3 After culturing for 2 - 3 days, centrifuge at 300g for 10 minutes, remove the supernatant, and add 2 mL of fresh complete medium to remove the T cell activation reagent. Then continue culturing at 37 °C under 5% CO₂.
1.4 Every 2 days, pipette the cell suspension thoroughly to mix evenly, add 2 times the volume of fresh complete medium (the ratio of the original volume to the supplementary volume is 1:2), and count the cells to calculate the amplification factor. Then culture them in a 37 °C, 5% CO₂ incubator. For example, if the medium is supplemented on day 4, and the original volume is 2 mL, 4 mL of medium should be added, and the final total volume will be 6 mL.
Precautions
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When incubating the T cell activation reagent with cells, it is necessary to mix them thoroughly to improve the activation efficiency.
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The titer of the T cell activation reagent can be adjusted within the range of 1:100 - 300.
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The time for centrifuging to remove the T cell activation reagent can be optimized according to customer requirements.
Additional Precautions
This product is only applicable to in vitro cell culture and cannot be directly used for clinical treatment.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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