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Introduction
Reliable separation and transfer of high-molecular-weight (HMW) proteins is often a major challenge for researchers. While 4–20% Tris-Glycine gels are commonly used for a broad range of proteins, large proteins tend to remain compressed near the top of the gel. For superior performance, Tris-Acetate gels provide a better solution. Operating at a near-neutral pH (~8.1), Tris-Acetate gels deliver enhanced resolution and improved migration for HMW proteins compared to Bis-Tris or Tris-Glycine systems. The lower acrylamide concentration facilitates smoother protein movement, resulting in higher transfer efficiency and greater detection sensitivity. Moreover, the neutral buffer environment minimizes protein modification, producing cleaner, sharper bands ideal for SDS-PAGE and Western blot applications.
Specification
| Package | 10 PCS |
| Full name | 3-8%Precast-Glgel Tris-Acetate PAGE 10well |
| Applications | SDS-PAGE, Native-PAGE, WB and other protein detection experiments |
| Summary | Tris-Acetate system precast gel is a ready-to-use, safe, fast, high-performance prefabricated polyacrylamide gel, using automatic glue pouring production technology, to ensure the high stability and repetition of product quality, the use of glass glue plate, can effectively reduce the non-specific adsorption of protein, make the protein band more sharp, clear, effective separation of 4-5 kDa large protein molecules, short electrophoresis time, at 15 V voltage, electrophoresis can be completed in 6 min, the gel does not contain SDS, It can be used for both denaturing and native electrophoresis and is compatible with most mini tanks on the market. |
| Features | Ready-to-use, high security,save time, high resolution,the strips are uniform,high stability, high compatibility, cost-effective. |
| Composition | 1 pcs gel |
| Gel Size | length 8.1 x width 7.4 x thickness .15 cm |
| Plate Size | length 9.8 x width 8.4 x thickness .41 cm |
| Buffer System | Tris-Acetate buffer system |
| Maximum Sample Loading Volume | 6 μl |
| Recommended Sample Loading Volume | 3 μl |
Storage
2-8°C
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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