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Product Description:
2xYT Broth is a liquid nutrient medium optimized for the high-density growth of E. coli and the efficient propagation of filamentous bacteriophages such as M13. This medium is an enhanced version of traditional YT, providing a richer formulation that supports vigorous bacterial cultures essential for phage display, plasmid production, and other molecular biology applications.
Unlike 2xYT Agar or 2xYT Top Agar, this broth contains no agar, making it ideal for suspension cultures where cells and phages grow in a well-mixed liquid environment.
Key Features:
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Liquid medium – no gelling agents, designed for shaking cultures.
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Supports robust growth of E. coli and maintenance of M13 bacteriophage.
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Higher nutrient concentration than standard YT broth for improved yield.
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Ideal for DNA plasmid preparation, phage production, and protein expression.
Composition (per liter):
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Yeast Extract: 10.0 g
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Tryptone: 16.0 g
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Sodium Chloride (NaCl): 5.0 g
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Usage: Dissolve 31.0 g/L in distilled water, autoclave at 121°C for 15 min.
Comparison with 2xYT Agar & 2xYT Top Agar:
| Product | Form | Agar Content | Usage |
|---|---|---|---|
| 2xYT Broth | Liquid | None | Suspension cultures of E. coli, M13 phage, plasmid production. |
| 2xYT Agar | Solid | 15 g/L | Plate cultures for E. coli and M13 phage growth on solid media. |
| 2xYT Top Agar | Semi-solid | 7 g/L | Soft agar overlay for phage plaque assays and M13 propagation. |
Specifications:
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Total Product Size: 250 g
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Packaging: 1 container (250 g)
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Shipping Conditions: Room Temperature (RT)
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UNSPSC Code: 41106200 – Microbiological Media
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When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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