2xYT Agar Medium (Dehydrated Powder)

Product Description:

2xYT Agar is a nutritionally rich solid medium specifically designed for the growth and maintenance of recombinant E. coli strains, as well as filamentous phages such as M13, commonly used in molecular biology research. This dehydrated powder formulation is ideal for preparing agar plates to support robust bacterial and phage propagation.

The enhanced composition of 2xYT Agar ensures optimal bacterial growth and efficient phage reproduction, making it suitable for applications including phage display, cloning, and plasmid amplification. Tryptone and Yeast Extract serve as comprehensive nutrient sources, supplying carbon, nitrogen, vitamins, minerals, amino acids, and growth factors essential for E. coli and phage viability. Sodium chloride maintains proper osmotic balance and electrolyte levels to support cellular transport processes.

Key Features:

• Optimized for E. coli infected with M13 bacteriophage.

• Supports high-density bacterial growth and phage replication.

• Ideal for recombinant DNA techniques and molecular cloning.

Typical Applications:

• Growth and maintenance of E. coli strains in molecular biology.

• Cultivation of filamentous phages such as M13.

• Preparation of solid medium plates for recombinant protein expression systems.

Composition (per liter):

• Tryptone: 16.0 g

• Yeast Extract: 10.0 g

• Sodium Chloride: 5.0 g

• Agar: 15.0 g

Preparation Instructions:

1. Dissolve 46 g of 2xYT Agar powder in 1 liter of distilled water.

2. Heat with frequent stirring to bring to a complete boil.

3. Autoclave at 121°C for 15 minutes.

4. Pour into sterile petri dishes under aseptic conditions.

pH: 6.8 ± 0.2 at 25°C

Product Code: [Insert Product Code]
Packing Size: [e.g., 500 g / 1 kg]

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