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Description
Reliable cell attachment, consistent cell growth, and reproducible experimental results are essential requirements in modern cell culture workflows. This 12 Well Cell Culture Plate is designed to provide a stable and controlled environment for adherent cell culture, cell-based assays, microscopy, and biotechnology research through a high-quality tissue culture treated (TC treated) polystyrene surface.
Manufactured from optically clear polystyrene (PS), this 12 well tissue culture plate features a flat bottom design that supports high-quality microscopic observation, fluorescence imaging, and live cell analysis. The TC treated surface improves surface wettability and promotes efficient protein adsorption, helping researchers achieve reliable cell attachment and uniform cell growth across wells.
Each sterile 12 well cell culture plate is gamma irradiated, DNase/RNase free, non-pyrogenic, and non-cytotoxic for sensitive biological applications. With a standard multiwell format, low-evaporation lid design, and clear well identification, this plate is suitable for routine cell culture, transfection studies, drug screening, cell viability assays, and high-throughput cell-based research workflows.
The ANSI/SBS compatible footprint allows integration with commonly used laboratory automation systems, imaging platforms, incubators, and liquid handling equipment, making it an ideal alternative for laboratories requiring consistent performance similar to premium 12 well tissue culture plates.
Specifications
| Product Type | 12 Well Cell Culture Plate |
| Material | Polystyrene (PS) |
| Surface Treatment | Tissue Culture Treated (TC Treated) |
| Well Format | 12 Wells (3 × 4 Array) |
| Bottom Shape | Flat Bottom |
| Sterilization | Gamma Irradiated Sterile |
| DNase/RNase | Free |
| Pyrogen | Non-pyrogenic |
| Cell Growth Area | 3.83 cm² / well |
| Maximum Well Volume | 6.9 mL / well |
| Recommended Working Volume | 1.5–2.2 mL / well |
| Well Dimension | D1 = 22.0 mm; D2 = 20.8 mm; H = 17.0 mm |
| Packaging | Individually Packed with Lid |
Features
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TC Treated Tissue Culture Surface: The hydrophilic surface improves cell attachment and supports consistent growth of common adherent cell lines.
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Flat Bottom Optical Design: Provides excellent transparency for bright-field microscopy, fluorescence imaging, and live cell observation.
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High Clarity Polystyrene Construction: Crystal-clear PS material enables easy visualization of cells and reliable laboratory handling.
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Sterile and Biocompatible: Gamma sterilized, DNase/RNase free, non-pyrogenic, and non-cytotoxic for reproducible cell culture workflows.
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Low Evaporation Lid Design: Condensation rings and vented lid structure help maintain humidity and improve gas exchange during incubation.
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Alphanumeric Well Identification: Clear well marking improves sample tracking and reduces handling errors during multi-condition experiments.
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Automation Compatible Multiwell Format: Standard footprint supports laboratory automation, imaging systems, and plate-based workflows.
Applications
This 12 well tissue culture plate is widely used in academic, biotechnology, pharmaceutical, and biomedical research laboratories:
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Adherent Cell Culture: Supports routine expansion and maintenance of mammalian cell lines including HEK293, CHO, HeLa, and other adherent cells.
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Cell Proliferation and Viability Assays: Suitable for cell growth analysis, CCK-8, MTT, live/dead staining, and cytotoxicity evaluation.
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Transfection Studies: Used for plasmid DNA, siRNA, and molecular biology transfection workflows requiring reliable cell attachment.
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Drug Screening and Cell-Based Assays: Provides independent wells for compound testing, dose response studies, and biological evaluation.
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Microscopy and Fluorescence Imaging: Flat bottom clear PS construction enables bright-field imaging and fluorescence microscopy applications.
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Immunofluorescence and Cell Staining: Suitable for cell fixation, staining, and imaging-based analysis.
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Protein Expression and Cell Signaling Research: Supports stimulation experiments, pathway analysis, and biotechnology research workflows.
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High Throughput Cell-Based Research: Compatible with plate readers, automated liquid handling, and laboratory screening platforms.
Why Choose 12 Well TC Treated Cell Culture Plates?
The 12 well cell culture plate provides an excellent balance between experimental throughput and individual well growth area. Compared with smaller well formats, each well offers a larger surface area of 3.83 cm², making it suitable for experiments requiring more cells, easier sample handling, and improved microscopic observation. The combination of TC treated surface, flat bottom design, sterile packaging, and high clarity polystyrene material provides reliable performance for cell culture and biological research applications.
Ordering Information
| Well Format | Surface Treatment | Bottom Shape | Packaging |
|---|---|---|---|
| 12 Wells | Tissue Culture Treated (TC Treated) | Flat Bottom | Sterile Individually Packed with Lid |
Frequently Asked Questions
What is a 12 well cell culture plate used for?
A 12 well cell culture plate is commonly used for adherent cell culture, cell expansion, drug screening, transfection studies, microscopy, cell viability assays, and other biological research applications requiring multiple independent culture conditions.
What is the culture area of a 12 well plate?
Each well of this 12 well tissue culture plate provides a growth area of 3.83 cm², allowing sufficient surface area for adherent cell attachment and expansion.
What is the maximum volume of a 12 well plate?
Each well has a maximum capacity of 6.9 mL. The recommended working volume depends on cell type, culture duration, and experimental requirements.
What is the difference between TC treated and non-treated cell culture plates?
TC treated plates have modified polystyrene surfaces with improved wettability, allowing better protein adsorption and supporting stronger attachment of adherent cells. Non-treated plates are mainly used when cell attachment is not required.
Can this 12 well plate be used for microscopy?
Yes. The clear flat bottom polystyrene design provides excellent optical clarity for bright-field microscopy, fluorescence imaging, and live cell analysis.
Is this 12 well tissue culture plate sterile?
Yes. The plate is gamma sterilized, DNase/RNase free, non-pyrogenic, and designed for direct use in sterile cell culture workflows.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

