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Description
Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.
Specifications
| Grade | Ultra Pure |
| Formula | C47H8N3NaO7S2 |
| MW | 854.03 |
| CAS | 6104-58-1 |
| Appearance | Dark brown to Violet-brown powder |
| Solubility (1mg/mi in Water) | Dark blue solution |
| Dye Content | ≥65% |
| Loss on drying | ≤7% |
| Residue on Ignition | ≤2% |
| Suitable for electrophoresis | Pass test |
| Storage | R.T |
Application
Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[3] It has also been used in Bradford protein assay.
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