0 item(s)
View cart
You have no items in your shopping cart.
$8.00
Sku: A610452-5g
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by July 04, 2026
Description
In protein electrophoresis, histology, and microscopy workflows, weak staining contrast, high background noise, and inconsistent dye binding often lead to unclear gel bands and unreliable quantitative interpretation. Researchers working with SDS-PAGE, IEF electrophoresis, and tissue section analysis require a stable, high-contrast biological stain that ensures reproducible visualization of proteins and cellular structures.
Fast Green FCF (CAS 2353-45-9) is a water-soluble triarylmethane biological stain designed for high-performance protein staining and tissue visualization. It binds strongly to proteins and provides excellent contrast in electrophoresis gels, histological sections, and microscopy imaging. Compared with conventional counterstains, it delivers improved signal clarity, low background interference, and consistent batch-to-batch performance for research applications.
This reagent is widely used in SDS-PAGE protein gel staining, IEF electrophoresis visualization, histology staining, and Masson trichrome counterstaining, supporting accurate qualitative and quantitative biological analysis in modern life science laboratories.
Specification
| Product Name | Fast Green FCF |
| CAS No. | 2353-45-9 |
| SKU | A610452 (Former: FB0452) |
| Molecular Formula | C37H34N2O10S3Na2 |
| Molecular Weight | 808.86 |
| Appearance | Red to brown powder |
| Solubility | 1 mg/mL in water (dark blue-green solution) |
| Assay (Dry Basis) | ≥85% |
| Storage | Room Temperature |
| Grade | Indicator / Biological Stain |
Features
- High-performance protein staining dye for SDS-PAGE and electrophoresis gel visualization with strong band contrast.
- Optimized for histological staining and microscopy imaging, delivering clear tissue differentiation and low background.
- Water-soluble formulation enables fast preparation of biological staining solutions in aqueous systems.
- Compatible with IEF electrophoresis and protein analysis workflows for research-grade reproducibility.
- Stable triarylmethane dye structure ensures consistent staining performance across batches.
Applications
- SDS-PAGE protein gel staining for visualization of protein expression and molecular weight distribution.
- IEF (isoelectric focusing) electrophoresis staining for protein separation and analytical profiling.
- Histology tissue staining for cytoplasmic and extracellular matrix visualization under microscopy.
- Masson trichrome counterstain as a replacement for traditional Light Green SF dyes.
- Protein quantification assays in biochemical and molecular biology research workflows.
- Cell structure imaging and microscopy contrast enhancement in biological samples.
Performance Advantages in Protein & Tissue Staining
Fast Green FCF is widely used as a benchmark protein staining reagent in electrophoresis and histology due to its strong affinity to proteins and excellent optical contrast.
-
Improved signal-to-background ratio in SDS-PAGE gels
-
Stable color development for microscopy imaging
-
Compatible with standard biochemical staining protocols
-
Reliable reproducibility in quantitative protein analysis
Documents
Frequently Asked Questions
What is Fast Green FCF used for in laboratory research?
Fast Green FCF is primarily used as a protein and tissue staining dye for SDS-PAGE electrophoresis, IEF protein separation, histology, and microscopy imaging applications.
Is Fast Green FCF suitable for SDS-PAGE gel staining?
Yes, it is widely used in SDS-PAGE protein gel staining to visualize protein bands with high contrast and low background interference.
Can Fast Green FCF be used for histology staining?
Yes, it is commonly used in histological staining procedures for tissue sections, especially for cytoplasmic and connective tissue visualization.
What makes Fast Green FCF different from other protein stains?
It provides strong protein binding, high contrast staining, and stable color performance, making it suitable for both electrophoresis and microscopy applications.
How should Fast Green FCF be stored?
Store at room temperature in a tightly sealed container, away from moisture and direct light to maintain stability.
Is this product suitable for diagnostic or clinical use?
No. Fast Green FCF is intended for laboratory research use only (RUO) and is not for diagnostic or therapeutic applications.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
